以金纳米粒子为探针可视化监测丝素蛋白水溶液中组装行为的研究

项目名称： 以金纳米粒子为探针可视化监测丝素蛋白水溶液中组装行为的研究

项目编号： No.51303124

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 贾兰

作者单位： 太原理工大学

项目金额： 25万元

中文摘要： 通过仿生的绿色化学方法获取高性能、多功能的丝基材料是近年来材料领域关注的热点，而对丝素蛋白在水溶液中组装过程的深入理解是实现这一目标的关键因素。目前对丝素蛋白组装机理的了解还不够完全，采用的研究方法难以直观监测组装过程以及表征高浓度丝素蛋白溶液。为此，本项目提出了以金纳米粒子为探针研究丝素蛋白水溶液中组装行为的新思路。 本项目拟选用含甘氨酸与丙氨酸的重复序列多肽作为丝素蛋白的简化模型，多肽末端的酪氨酸原位还原的金纳米粒子作为固定在多肽模型上的探针。通过溶液颜色以及紫外吸收峰的改变，研究外源性因素对多肽溶液组装行为的影响规律，建立以金纳米粒子为探针研究多肽在水溶液中组装行为的方法；进而通过模拟家蚕丝腺内的溶液微环境，探索高浓度丝素蛋白在水溶液中的组装规律。本项目将为研究丝素蛋白的溶液组装行为提供了一种简单直观的新方法，有利于组装规律的深入理解，并为人工制备新型丝基材料提供理论指导。

中文关键词： 金纳米粒子；探针；丝素蛋白；多肽；组装行为

英文摘要： In recent years, much attention has been paid to artificially preparation of high-performance and multi-functional materials from reconstituted silk proteins by biomimetic green process in materials research field. In order to reach the goal, it is essential to understand the self-assembling process of silk protein in aqueous solution. However, the underlying assembly mechanisms of silk proteins remains incompletely understood. A variety of methods have been developed to investigate the assembling process, however, most of them are difficult to monitor the assembly behavior directly and characterize the concentrated solution of silk fibroin. Gold nanoparticles(Au NPs) has been widely used for developing various chromatic sensors based on the reversible red-blue color changes between dispersion-aggregation states. Therefore, a new approach for sensing assembly behavior of silk fibroin in aqueous solution is expected to construct with in-situ Au NPs as sensitive indicator. Peptide composed of repetitive glycine-alanine, which obtained from the hydrolysate of Bombxy mori silkworm silk fibroin, is planned to use as simplified model of silk fibroin. Tyrosine residue is able to reduce Au(III) ions to Au NPs in situ and further stabilize them without any external reducing or capping reagents. The conformational chang

英文关键词： Gold nanoparticles；Indicator；Silk fibroin；Peptide；Assembly behavior