具有互贯网络结构的高强度微纤化纤维素/胶原复合水凝胶的构建与结构调控

项目名称： 具有互贯网络结构的高强度微纤化纤维素/胶原复合水凝胶的构建与结构调控

项目编号： No.51473150

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 一般工业技术

项目作者： 郑学晶

作者单位： 郑州大学

项目金额： 80万元

中文摘要： 本申请拟构建具有互贯网络结构的微纤化纤维素/胶原复合水凝胶，通过复合水凝胶的结构调控达到性能可控的最终目的。具体地，拟从动物皮中提取出胶原，配置成胶原溶液；用预处理和高压均质作用相结合的方法使纤维素微纤化，制备出长径比极大的纳米微纤化纤维素（MFC），用冷冻干燥法构建MFC毡的三维精细网络结构。将MFC毡加入胶原溶液中，在一定条件下使胶原自组装，形成具有三股螺旋结构的胶原微纤维；用低生物毒性的交联方法使胶原交联形成网络结构；控制交联和复合条件，使在形成复合水凝胶的过程中，MFC网络和胶原网络达到高度的相互贯通。极大的长径比和网络结构赋予了纳米MFC优异的增强效果，而高度贯通的双网络结构使复合水凝胶的机械强度大幅度提高。深入研究复合水凝胶的微结构、聚集态结构与性能之间的关系，设计出具有优异生物相容性、良好生物降解性和机械强度大幅度提高的全生物质高强度复合水凝胶，有望应用于人工软骨组织工程。

中文关键词： 胶原；微纤化纤维素；复合水凝胶；结构调控；组织工程

英文摘要： This application aims to construct a high mechanical strength microfibrillated cellulose/collagen biomass composite hydrogel with interpenetrating network structure. Collagen will be extracted from animal skin. Microcrystal cellulose (MCC) will be used to prepare microfibrillated cellulose (MFC). MCC will be pre-treated with alkali or enzyme, then microfibrillated by using a high pressure homogenizer. The fibers of MCC will be separated into nano microfibrils with diameters of about 20-30 nm while length of microns. MFC mat with fine three-dimensional network structure will be prepared by lyophilization and immersed in collagen solution. Under proper conditions, the collagen peptide macromolecular chains will self-assembly into collagen fibrils with triple-helical structure. Collagen will be crosslinked using crosslinking agents with low biological toxicity, and a collagen network will be formed throughout the MFC network. During this process, a MFC/collagen composite hydrogel with highly interpenetrating network structure will be formed. By adjusting the chemical strucutre, macromolecular chain entanglement and aggregation, a composite hydrogel with desired pore size, pore shape, pore structure and porosity will be prepared. The extremely high aspect ratio endows MFC with super reinforcing effect, and the interpenetrating network structure results in drastically improved mechanical properties. Due to the good biocompatibility, biodegradability as well as enhancement in mechanical strength, the composite hydrogel in this application might provide a novel substitute as a scaffold for tissue engineering of cartilage.

英文关键词： collagen;microfibrillated cellulose;composite hydrogel;structure regulation;tissue engineering