拟南芥对二氧化氮胁迫应答中水杨酸的调节机理

项目名称： 拟南芥对二氧化氮胁迫应答中水杨酸的调节机理

项目编号： No.31270446

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 郝林

作者单位： 沈阳师范大学

项目金额： 78万元

中文摘要： 二氧化氮（NO2）是主要的空气污染物之一，认识植物对NO2的应答机理具有重要的科学意义与指导生产实践的价值。我们前期研究显示，内源水杨酸（SA）水平及信号转导是拟南芥耐受高浓度NO2胁迫所必需的，然而，对其分子作用机理几乎一无所知。本项目以拟南芥SA高积累和缺失突变体、信号转导阻断突变体以及相互间的遗传组合为材料，通过双向凝胶电泳和基质辅助激光解析电离时间飞行质谱分析，并结合基因表达定量分析，阐明拟南芥对NO2胁迫应答的蛋白质生物学功能，揭示SA在拟南芥对NO2胁迫应答中的调节机理，为利用植物修复环境污染提供科学依据。拟解决的关键科学问题是：植物对NO2胁迫应答的蛋白质生物学功能以及SA的调节作用、SA对蛋白质磷酸化以及某些具有重要生物学功能的蛋白质时空表达特异性的调节及其在NO2胁迫应答中的作用。到目前为止，相关的研究尚未见报道。在SCI期刊发表不少于4篇论文,并培养4名以上硕士研究生。

中文关键词： 拟南芥；蛋白组学；二氧化氮；水杨酸；一氧化氮

英文摘要： Nitrogen dioxide (NO2), one of the main traf?c-related air pollutants and precursors of photochemical smog and ground-level ozone, is currently under intensive investigation. Although visible injury due to NO2 is very rare in the ?eld where NO2 is at a ppb level, growing evidence shows that ambient NO2 has caused reduction and deterioration in crop and vegetable yield and quality in some regions on the world, especially in greenhouses where NO2 at a ppm level may induce visible injury in some vegetables. In previous studies, we found that endogenous SA and its signaling were prerequisite to improve plant tolerance to NO2 exposure,and exogenous application of SA also protect plants against NO2 damage. However, our knowledge of SA-related molecular mechanisms responsible for plant tolerance to NO2 exposure still remains largely limited. To assess the molecular mechanism of endogenous SA protecting plants against NO2 stress, proteomic studies combined with temporal and spatial expression analyses of related genes involved in plant response to NO2 are performed on SA-altering Arabidopsis mutants including high SA accumulation, SA deficiency, SA signaling blockage, as well as their hybridization combinations. The total proteins from NO2-exposed plants and their NO2-unexposed parallels are separated by two-dimensional

英文关键词： Arabidopsis thaliana；Proteomics；Nitrogen dioxide；Salicylic acid；Nitric oxide