基于微流控的神经干细胞与内皮细胞三维微环境构建及相互作用机制研究

项目名称： 基于微流控的神经干细胞与内皮细胞三维微环境构建及相互作用机制研究

项目编号： No.31300809

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 葛丹

作者单位： 大连理工大学

项目金额： 23万元

中文摘要： 目前用于体外研究神经干细胞增殖与分化机制的细胞模型主要采用细胞二维培养方式，忽略了神经干细胞微环境（niche）中细胞与细胞间、细胞与细胞外基质间的相互作用影响。为此本项目首次提出一种基于微流控构建神经干细胞三维微环境的新方法，并创新性地开展了神经干细胞与血管内皮细胞在三维微环境中相互作用机制的研究。重点开展微流控芯片上神经干细胞与血管内皮细胞间隔式接触共生长模型的构建，神经干细胞与血管内皮细胞的无血清共生长条件的筛选与优化，和上皮衍生因子（PEDF）在血管内皮细胞存在条件下促进神经干细胞增殖与分化的机制研究。项目成果对于研究哺乳动物神经系统的发育、神经组织临床移植以及体外药物筛选等具有重要的理论意义和实际应用价值。

中文关键词： 神经干细胞；微流控芯片；三维微环境；电化学检测；

英文摘要： Two-dimension cell culturing has been the most widely used research method for the in-vitro study of neural stem cells proliferation and differentiation. However,two-dimension method fails to consider the important cell-cell and cell-matrix interactions in niche. This study proposed a novel protocol based on microfluidics that better simulates the three-dimension niche environment. This study focused on the interaction mechanism between neural stem cells and brain vascular endothelial cells in three-dimension symbiotic conditions, which includes the study of contacting alternative co-growth of neural stem cells and vascular endothelial cells on microfluidic chips,the screening and optimization of the non-serum co-culturing conditions of neural stem cells and vascular endothelial cells, and the study of molecular mechanisms by which pigment epithelium derived factor (PEDF) promotes the proliferation and differentiation of neural stem cells under the condition of the brain vascular endothelial cells exist. This work would provide great value in theory and practical application for promoting the studies of mammalian nervous system development, clinical transplantation of nervous tissues, and drug screening.

英文关键词： Neural stem cells；Microfluidic chips；Three-dimension niche；Electrochemical detection；