胶体微粒的结构和表面性质对胞吞和细胞功能的调控

项目名称： 胶体微粒的结构和表面性质对胞吞和细胞功能的调控

项目编号： No.50873087

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 高长有

作者单位： 浙江大学

项目金额： 36万元

中文摘要： 围绕细胞层次上调控胶体微粒与细胞相互作用的需要，制备了可降解的PLGA微粒和不可降解的SiO2微粒、具有细胞亲和表面和抗细胞亲和表面的PLGA和SiO2胶体微粒，以及中空的聚电解质微胶囊，发现了微胶囊的结构演化现象并制备了具有微纳结构的胶体微粒，表征了胶体微粒的结构和性能。以内皮细胞、平滑肌细胞和肝细胞为模型研究并阐明了胶体微粒的胞吞过程、速率、途径和细胞内分布规律；考察了细胞吞噬胶体微粒后细胞活性、增殖、迁移和细胞外基质分泌等功能的变化规律。作为合作伙伴获得了欧盟第七轮框架协议项目和玛丽居里人才交流计划项目；申请并获得了国家自然科学基金重大国际合作项目的进一步资助。发表SCI收录论文20篇，其中ACS Nano（IF 9.8）1篇、Biomaterials（IF 7.8）3篇、其他IF3以上论文7篇；应邀在Journal of Materials Chemistry撰写综述一篇；国际会议大会报告1人次、邀请报告3次，其他报告16人次；授权发明专利5项。出站博士后1人，培养博士研究生5人。与西班牙生物材料研究所、德国马普胶体与界面研究所等单位开展了学术交流活动。

中文关键词： 胶体微粒；界面；生物材料；胞吞；细胞功能

英文摘要： In order to investigate the interactions between colloidal particles and cells in a cellar and subcellular level, several types of colloidal particles were prepared, including degradable PLGA and nondegradable SiO2 particles with different affinity to cells, and hollow polyelectrolyte microcapsules. Evolution of microcapsules to 1D nanorods and nanotubes was observed and its mechanism was elucidated. Based on this new phenomenon particles having a micro-nano structure were obtained. The cellular uptake processes, rates, pathways and intracellular distribution of the colloidal particles in endothelial cells, smooth muscle cells and hepatocytes were then investigated to correlate the principles of particle-cell interactions. Influences of cellular uptake of the particles on cytoviability, proliferation, migration and secretion of extracellular matrix were clarified too. Based on these results, as a partner we are participating in a European commission project and Marie Curie exchange program; and our proposal on the next study is supported by NSFC as a Major International (Regional) Joint Research Project. 20 papers were published in SCI-cited journals, with 1 in ACS nano, 3 in Biomaterials and 3 in those journals with impact factors >3, and 1 in Journal of Materials Chemistry as a highlight article. The results were reported in international conferences as 1 plenary and 3 invited lecture. 5 Patents were authorized. 1 Postdoc finished her work, and 5 students received their Ph.D. Academic communication was carried out with famous institutions such as CIC BioMagune, and Max-Planck-Institute of Colloids and Interfaces.

英文关键词： olloidal particles; interface;