MT1-MMP介导bFGF诱导的hESC-MSC脂肪分化作用及其机制研究

项目名称： MT1-MMP介导bFGF诱导的hESC-MSC脂肪分化作用及其机制研究

项目编号： No.81501943

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 宋兴辉

作者单位： 浙江大学

项目金额： 18万元

中文摘要： 每年都有大量的人需要进行脂肪组织移植，脂肪组织工程技术的应用带来新的治疗模式，探索进行高效脂肪分化和再生一直是脂肪组织工程中关键的核心问题。我们前期研究结果显示，人胚胎干细胞来源的间充质干细胞（hESC-MSCs）在碱性成纤维细胞生长因子（bFGF）作用下能够高效率的向脂肪分化，并且显著缩短诱导时间。结合文献信息和我们重要发现的基础上，本课题采用hESC-MSCs作为脂肪组织工程的种子细胞，复合自主研发的PLGA支架，建立体内外脂肪分化和脂肪组织工程模型，研究bFGF促进hESC-MSCs（二维体系和三维体系）脂肪分化和体内脂肪组织再生的潜能，并且进一步阐明基质金属蛋白酶（MT1- MMP）在bFGF促进hESC-MSCs脂系分化中的调控机制。本研究结果将可能为今后提高临床组织工程脂肪移植效率和修复效果，减少患者等待移植时间提供理论依据和实验基础，造福广大脂肪组织缺损患者和整容整形需求者。

中文关键词： 生长因子；基质金属蛋白酶；间充质干细胞；诱导分化；再生医学

英文摘要： Every year there are a lot of people need to transplantation of adipose tissue and adipose tissue engineering application brings new treatment mode, explore effectively adipose differentiation and regeneration has always been the core of the key issues in the adipose tissue engineering. Our preliminary results show that human embryonic stem cells between the source of mesenchymal stem cells (hESC - MSCs) in alkaline fibroblast growth factor (bFGF) under the action of can high efficiency to adipose differentiation, and significantly shorten the induction time.Combined with literature information and important, we find that, on the basis of this topic using hESC - MSCs as fat tissue engineering seed cells, the PLGA scaffolds of compound independent research and development, differentiation and fat fat tissue engineering in vivo and in vitro model, research bFGF promote hESC - MSCs ( system of two-dimensional and three-dimensional) adipose differentiation and the potential of adipose tissue in the body, and further clarify matrix metalloproteinases (MT1 -MMP) in bFGF promote the regulating mechanism in the hESC - MSCs adipose differentiation.This study could improve the efficiency of clinical tissue engineering of fat transplantation for the future and repair effect, reduce the time of patients waiting for transplantation provides theoretical basis and experimental base, the benefit of the adipose tissue defect patients and cosmetic plastic demanders.

英文关键词： basic fibroblast growth factor;matrix metalloproteinase;mesenchymal stem cells;inducing differentiation;regenerative medicine