植物抗朱顶红褪绿环斑病毒免疫应答机制研究

项目名称： 植物抗朱顶红褪绿环斑病毒免疫应答机制研究

项目编号： No.31471828

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 刘雅婷

作者单位： 云南农业大学

项目金额： 80万元

中文摘要： 朱顶红褪绿环斑病毒(HCRV)是2013年在中国云南首次发现的番茄斑萎病毒属新种。该病毒一经报道，就在番茄、烟草等多种经济作物上被发现，流行速度极快，已成为我国南方优势种群，对农业生产造成了巨大损失，亟待研发有效的控制技术，探明植物寄主免疫机制是防治该新病毒的基础。本项目组对该病毒进行了研究，首次报道了该病毒的全基因组序列。课题以HCRV为研究对象，以TSWV为对照材料，进行寄主植物抗病毒机制的比较研究。应用分子生物学、生物化学和生物信息学等现代技术，探究HCRV入侵引起植物寄主在small RNA差异表达、siRNA含量和丰度变化，以及蛋白质泛素修饰种类和位点差异等响应机制，从表观遗传学层面解析植物抵抗HCRV的免疫应答和调控机制，为基因工程水平改良农作物、提高农作物抗病毒能力提供有力的理论依据。拟发表论文6-8篇，其中SCI刊源文章2篇以上，申请专利2项。

中文关键词： 表观遗传学；朱顶红褪绿斑病毒；小RNA；泛素化；番茄斑萎病毒属

英文摘要： Hippeastrum Chlorotic Ringspot Virus (HCRV) which was first found in Yunnan in 2013 belongs to Topsovirus.After it is reported, the virus was found on tomato, tobacco and other crops. Further study found that this virus has spread out quickly and has caused huge losses in agriculture. It has become the dominant species in South China. It is necessary to develop effective control technologies and clear plant host immune mechanism is the basis for prevention and treatment of this new virus. The project team has reported the complete genome sequence of the virus in the previous study. In the study, HCRV is the research subject virus and TSWV is the check virus. The research will use molecular biology, biochemistry, and bioinformatics tools to reveal differences in expression of small RNA，changes of siRNA content and abundance,the protein ubiquitin modification type and site difference in plant hosts. The results will reveal immune response and regulation mechanism of plants to HCRV based on Epigenetics and provide strong theoretical basis for improvement of crop resistance by genetic engineering.This project aims to publish 6-8 papers and apply for 2 patents.

英文关键词： Epigenetics;Hippeastrum chlorotic ringspot virus;small RNA;Ubiquitination;Tospovirus