miR-21抑制PTEN介导心脏衰老

项目名称： miR-21抑制PTEN介导心脏衰老

项目编号： No.81670366

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 医药、卫生

项目作者： 林胜辉

作者单位： 华侨大学

项目金额： 25万元

中文摘要： 心脏衰老是引起多种心脏疾病的主要原因，微小RNA广泛参与心血管疾病的各种病理生理过程，但它在心脏衰老中的作用尚未完全明确。我们的前期研究发现，微小RNA-21在细胞水平和动物水平的多种心脏衰老模型中均显著升高，过表达微小RNA-21可以诱导新生大鼠心肌细胞衰老。在本次申请课题中，我们拟基于已建立的细胞和动物心脏衰老模型，通过功能获得性和缺失性实验，明确微小RNA-21与心脏衰老的关系。此外，基于前期已鉴定出的靶基因PTEN，我们将通过细胞水平的功能挽救实验，明确微小RNA-21介导心脏衰老的分子基础，为心脏衰老的防治提供新的方法。

中文关键词： 微小RNA；心脏衰老；PTEN

英文摘要： Aging is among predominant risk factors for cardiovascular diseases. MicroRNAs (miRNAs, miRs) participate in many physiological and pathological processes in the cardiovascular system. However, the role of miRNAs in cardiac aging has not been completely understood. Our preliminary data showed that miR-21 was upregulated in multiple types of cardiac aging models both in vivo and in vitro. Overexpression of miR-21 could induce aging of the neonatal rat cardiomyocytes. In this project, we will further investigate the role of miR-21 in cardiac aging by gain-of-function and loss-of-function experiments both in vitro and in vivo. Based on the target gene PTEN that we have identified, rescue experiments will be performed to fully prove the role of PTEN in miR-21 mediated cardiac aging. This project will provide a novel therapy for cardiac aging.

英文关键词： MicroRNA;Cardiac aging;PTEN