脱氧雪腐镰刀菌烯醇诱导仔猪神经损伤的分子机制研究

项目名称： 脱氧雪腐镰刀菌烯醇诱导仔猪神经损伤的分子机制研究

项目编号： No.31472250

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 王希春

作者单位： 安徽农业大学

项目金额： 78万元

中文摘要： 脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）是由镰刀菌属病原真菌产生的次级代谢产物之一，广泛存在于农产品和饲料中，被世界卫生组织确定为最危险的自然发生食品污染物，对人类和动物的危害巨大。研究表明，DON是一种神经毒性物质，然而由于神经系统的复杂性，目前DON引起动物神经毒性的作用机制仍不完全清楚。本项目以对DON最敏感的仔猪为研究对象，拟运用RT-PCR、流式细胞术、ELISA、HPLC、单细胞凝胶电泳、体外神经细胞培养等技术，研究DON染毒猪脑组织与培养的神经细胞中DON的蓄积量、钙稳态、神经递质分泌、脂质过氧化、DNA损伤及细胞凋亡等的变化，结合神经组织和细胞超微结构及神经纤维缠结的观察结果，旨在从细胞和整体水平上揭示DON诱导仔猪神经损伤的分子机制，为DON神经毒性危害的解除提供理论依据。

中文关键词： 脱氧雪腐镰刀菌烯醇；仔猪；神经损伤；细胞凋亡

英文摘要： Deoxynivalenol(DON) is one of the secondary metabolites of the Fusarium and extensively existed in agricultural products and feed, determined as one of the most dangerous natural food contaminants by the WTO. DON has a great damage to human beings and animals, and studies have shown that DON is a neurotoxic substance, while the mechanism of neurotoxicity is unknown because of the neural complexity.In our study, the piglets, the most sensitive experiment animals to DON, are selected as the research object, and RT-PCR, flow cytometry, ELISA, HPLC, single cell gel electrophoresis, neuronal cells cultures in vitro are used in this test. In order to reveal the molecular mechanism of nerve injury induced by DON in cellular and overall level, the concentration of DON in brain tissues and nerve cells, calcium homeostasis, neurotransmitter secretion, lipid peroxidation, DNA damage and apoptosis will be studied, combined with the observation of nerve tissue and cell ultra microstructure and nerve fiber tangles. The research results of this project will provide a theoretical basis to remove DON neurotoxicity.

英文关键词： deoxynivalenol;piglet;nerve injury;apoptosis