胃俞募配穴调节胃运动的边缘系统整合及其分子机制研究

项目名称： 胃俞募配穴调节胃运动的边缘系统整合及其分子机制研究

项目编号： No.81473784

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 申国明

作者单位： 安徽中医药大学

项目金额： 72万元

中文摘要： 俞募配穴法是针灸临床常用有效配穴方法之一。本项目组前期研究结果表明胃俞募配穴调节胃运动具有协同效应，且胃俞募穴针刺信息在延髓迷走孤束复合体（DVC）和下丘脑室旁核（PVN）汇聚整合，通过迷走神经参与对胃运动的调节作用。边缘系统有内脏脑之称，是内脏活动的重要调节中枢，在调节胃运动方面也起着重要作用。本课题以胃俞募配穴调整胃运动与边缘系统的关系为切入点，通过功能磁共振成像技术,分析以边缘系统为重点的各个脑区的活动变化；在体微透析技术动态分析海马透析液中谷氨酸（Glu）含量变化；微陈列电极记录海马神经元放电；免疫荧光、western blotting及实时荧光定量PCR等技术检测海马和胃窦组织中NMDAR及其亚单位NR的表达。从临床和实验研究双层次开展针刺胃俞、募穴信息的边缘系统整合及其整合的分子生物学机制研究，为胃俞募配穴协同效应提供生物学依据。

中文关键词： 胃俞募配穴；边缘系统；海马；NMDAR；fMRI

英文摘要： The method of back-shu and front-mu points combined is one of the effective methods of clinical acupuncture. We discovered that the combined EA stimulation of gastric Shu and Mu points has synergistic efects, and the acupuncture signals of Shu and Mu points gather in DVC and PVN,gastric Shu and Mu points combination regulating gastric motility depended upon an intact vagus nerve. Limbic system is called visceral brain, it is an important central regulation of visceral activities,so it plays an important role in the regulation of gastric motility.In this study, we performed EA stimulation of the gastric Front-Mu Points (RN12) and gastric Back-Shu Points (BL21) to investigate the regulation of gastric motility as well as the relationship with limbic system,using the technology of functional magnetic resonance imaging,analysing the activity of different brain areas in the limbic system;Using the vivo microdialysis technique to microdialysis dynamic analysisthe content of glutamate in hippocampus; and using immunofluorescence, western blotting and real-time fluorescence quantitative PCR technique to detection the expression of NMDAR and the subunit NR in hippocampus and the gastric antrum . We study the information of gastric Shu and Mu points integrated in limbic system and the molecular biology mechanism of limbic system integration from the clinical and experimental research , providing a biological basis for the synergistic effect of gastric back Shu and front Mu points.

英文关键词： gastric back shu and front mu points combined;limbic system;hippocampus;NMDAR;fMRI