基于高灵敏腔体微电极阵列的抗肿瘤药物筛选新型传感器

项目名称： 基于高灵敏腔体微电极阵列的抗肿瘤药物筛选新型传感器

项目编号： No.61501039

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 无线电电子学、电信技术

项目作者： 刘儒平

作者单位： 北京印刷学院

项目金额： 21万元

中文摘要： 抗肿瘤药物体外筛选对癌症治疗和药物研究极为重要，基于微纳电极阵列的阻抗传感器因能实时、动态、持续地反应细胞活性状态，有望实现抗肿瘤药物高通量筛选。但现有阻抗传感器由于电阻梯度和电荷密度不可控，在结构和性能上造成抗干扰能力差和灵敏度低。针对这一问题，本项目将微纳电极阵列、纳米材料修饰、等离子谐振等技术与传感器结合，开展基于高灵敏腔体微电极阵列的抗肿瘤药物筛选新型传感器研究。重点研制一种具有纳米结构的三维腔体微电极阵列传感器，通过调节模板参数和凝胶层厚度对腔体电阻梯度实现有效控制，结合表面等离激元效应来调控电极表面电荷密度；研究该传感器的检测性能及传感机理，并以乳腺癌细胞为检测模型来探索其用于细胞活性检测及药物筛选的可行性，进而建立用于抗肿瘤药物筛选的传感器新理论和新方法，为未来研究模块化药物筛选微电极阵列提供传感器基础和技术支撑，对实现高通量抗肿瘤药物体外筛选具有重要的科学意义和应用价值。

中文关键词： 生物传感器；微电极阵列；表面处理；腔阵列；抗肿瘤药物筛选

英文摘要： In-vitro screening for anticancer drug is important for cancer treatment and drug discovery, and the microelectrode-based impedance biosensor gives a very promising method for real-time, dynamic, continuous activity monitoring. However, these biosensors have some disadvantages, such as low sensitivity and anti-interferential capability, which is due to uncontrolled resistance gradients and charge density. To this end, a sensing device will be developed using microelectrode arrays, coupled with nanomaterials technology and surface plasma resonance. We will focus on the development of 3D arrays constructed by gold nanoparticles and mesoporous materials, which exhibits surface plasmon polaritons that can be applied to control charge density. The resistance gradient will be adjusted by template parameters and gel-layer thickness. Also, the sensing performance and mechanism will be explored in terms of sensitivity, anti-interference and biocompatibility. Furthermore, breast cancer cells will be used as a model to investigate its practical applications of sensors. These results will allow for the development of new theory and methodology of biosensors for anticancer drug screening. As such, this study will provide information to better evaluate modularized microelectrode arrays for drug screening in the future. This study will be of great importance in the development of high-throughput biosensors for in-vitro anticancer drug screening.

英文关键词： biosensor;microelectrode array;surface processing techniques;cavity array;anticancer drug screening