具有主客体三级纳米结构的稀土配合物荧光编码微球的制备及生物检测应用

项目名称： 具有主客体三级纳米结构的稀土配合物荧光编码微球的制备及生物检测应用

项目编号： No.51502027

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 一般工业技术

项目作者： 张吉喜

作者单位： 重庆大学

项目金额： 21万元

中文摘要： 稀土配合物独特的大Stokes位移和窄带发射荧光特性使其在基于荧光编码的多指标分析生物检测中具有的巨大应用潜力。但是，在编码微球内可控地负载不同发光特征的稀土配合物，实现编码元素稳定、高亮度地发光，并精确调控编码荧光，仍是发挥其潜力面临的主要挑战。在此背景下，本项目拟结合介孔氧化硅微球（MSMs）和半导体聚合物对稀土配合物荧光性能提升各自的优势，通过稀土配合物在小尺寸（<10 nm）半导体聚合物点（Pdots）内、以及Pdots在大孔径（15-20 nm）MSMs内的有机无机材料复合，制备新型荧光编码微球。通过物理吸附-化学偶联结合的装载方法，实现Pdots客体颗粒在MSMs介孔孔道内的大量稳定装载，构建得到一种稀土配合物@Pdots@MSMs的主客体三级结构。最后，通过精确控制和装载不同颜色和比例的Pdots在MSMs主体微球中来实现荧光编码，并通过模式抗原的多指标检测阐释其应用潜力。

中文关键词： 纳米复合材料；主客体结构；稀土配合物；荧光编码微球；多指标检测

英文摘要： Lanthanide complexes possess a great application potential in the field of multiplexed biological detection based on fluorescence encoding, owing to their unique luminescence properties including large Stokes shift, and narrow emission peaks. However, main challenges towards fulfilling the potential still remain in terms of stable luminescence and high brightness of the encoding elements, fine-tuning of the encoding fluorescence by controllable loading of different lanthanide complexes in the encoding microspheres. Under this background, we plan to combine the advantages of mesoporous silica microspheres (MSMs) and semiconducting polymer in the enhancement of lanthanide complexes’ luminescence properties in the preparation of novel fluorescence encoded microspheres. The preparation is based on the organic-inorganic composition of lanthanide complexes in small-sized (<10 nm) semiconducting polymer dots (Pdots), and Pdots in large-pore (~15-20 nm) MSMs. A host-guest tertiary structure (lanthanide complex@Pdots@MSMs) will be constructed by large and stable loading of the guest Pdots in the mesopores of the host MSM through a combined method of physical adsorption and chemical conjugation. The encoding of the luminescence microspheres will be carried out by controllable loading of Pdots with varying color and ratio. Finally, the application potential of the encoded microspheres will be demonstrated by multiplexed detection of model antigens.

英文关键词： nanocomposite materials;host-guest structure;lanzhanide complex;fluorescence encoded microspheres;multiplexed detection