不同CO2浓度调控蛋白核小球藻CCM的分子机制

项目名称： 不同CO2浓度调控蛋白核小球藻CCM的分子机制

项目编号： No.31300295

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 范建华

作者单位： 华东理工大学

项目金额： 23万元

中文摘要： 微藻生长快，其固碳效率是植物的10-50倍，且微藻的应用面广价值高，因而微藻固碳已成为近年来国际上碳减排领域中的研究热点。探索微藻CCM（CO2浓缩机制）的调控机理，提高固碳效率是微藻固碳和微藻能源领域亟待研究的关键科学问题。 本项目拟以工业用高产油蛋白核小球藻为研究对象，以申请者课题组前期首次自主完成的该藻株全基因组信息为依托，首先研究该藻株在不同CO2浓度下光自养生长的生理生化表型特征；在此基础上，运用高通量转录组测序并耦合基因组信息，分析低/高CO2浓度下该藻株生长过程的全局基因转录图谱，挖掘关键基因和差异表达途径；同时，从计算预测、比较基因组分析、过表达实验验证等方面入手，探索不同CO2浓度调控小球藻CCM的分子机制。本项目对该藻CCM调控机制的研究，不仅对揭示微藻固碳机制具有重要的理论意义，而且可为进一步构建能够实现高效固碳和产油的能源微藻细胞工厂奠定基础。

中文关键词： 蛋白核小球藻；固碳机制；基因表达；转录组测序；基因工程

英文摘要： Compared with higher plants, microalgae can take the advantages of rapid growth rate, high biomass productivity, high value bio-products output as well as 10-50 times higher of carbon sequestration efficiency. Therefore, microalga-mediated CO2 fixation and biofuel production has become a hot topic in recent years in the field of carbon reduction. However, the issue of efficiently capturing CO2 and its molecular foundation with high growth rate is still a key challenge. Here we proposed a project to reveal the molecular mechanism of algal CCM regulating the expression and co-regulatory of CO2 bio-fixation network as well as key differentially expressed genes via biology techniques, such as comparative genomics, transcriptomics, algological physiology and genetic engineering. We developed the oleaginous Chlorella as a model to investigate the molecular foundation of photosynthetic growth process and that of rubust carbon bio-fixation, by presenting the foundation of whole-genome sequence of industrial strain Chlorella pyrenoidosa. The results in the project will help us to understand the molecular mechanism of alga CCM and genes regulating the process of photosynthetic CO2 fixation, and also lay the groundwork for the construction of cell factory to efficiently converse CO2 into biomass and biofuels.

英文关键词： Chlorella pyrenoidosa；Carbon fixation mechanism；Gene expression；Transcriptome sequencing；Genetic engineering