iPSc复合纳米支架材料定向诱导分化与移植治疗视网膜色素变性的研究

项目名称： iPSc复合纳米支架材料定向诱导分化与移植治疗视网膜色素变性的研究

项目编号： No.31300810

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 计菁

作者单位： 上海交通大学

项目金额： 23万元

中文摘要： 临床上治疗视网膜色素上皮细胞萎缩缺乏有效手段，细胞移植目前是该类疾病治疗中最具前景的方法，但迄今为止在提高种子细胞的诱导效率和移植细胞的存活方面仍存在不足，种子细胞定向分化的作用机理也有待于进一步探讨。我们的预实验结果显示视网膜色素上皮细胞（RPEc）注射移植能够恢复部分受损色素上皮层的功能，同时诱导性多能干细胞（iPSc）通过定向诱导分化可以获得RPEc，与CS-PCL/PCL纳米纤维支架复合后RPEc生长良好。因此本研究拟将iPSc定向诱导获得目标细胞后，复合CS-PCL/PCL纳米纤维支架形成组织片应用于移植治疗，并初步探索mir-124、mir-9、mir-200b在调控iPSc定向分化过程中的作用。本研究将iPSc作为种子细胞，CS-PCL/PCL纳米纤维作为支架，利用组织工程的手段，将探索一种更为高效的干细胞定向诱导的方法，为治疗该类眼底疾病的临床研究提供实验基础和新思路。

中文关键词： 视网膜色素上皮细胞；纳米材料；组织修复；诱导分化；细胞移植

英文摘要： The lack of effective means of treatment of retinal pigment cells of the retinal disease clinical cell transplantation is currently the most promising research in the treatment of such diseases, but so far there is still insufficient to improve cell induction efficiency and graft survival, its mechanism of actionneed to be further explored. Our pre-experiment results show that injection of retinal pigment epithelial (RPE) cells transplant can restore partially damaged pigment epithelium induced pluripotent stem cells (iPS) CS-PCL/PCL nanofiber scaffold complexes induced by directional differentiation RPE cells and growing well. To this end we propose assumptions: the iPS cells the composite CS-PCL/PCL nanofiber scaffold and directed inducing pieces of tissue containing the target cells used in transplantation in the treatment of, and explored the role of microRNA in regulation directed differentiation of iPS cells process. In this study, as seed cells, iPS cells CS-PCL/PCL nanofibers as a scaffold, using methods such as tissue engineering, will explore a more efficient stem cells induced for the treatment of a class of retinal disease clinical research experimental basis and new ideas.

英文关键词： retinal pigment epithelium cell；nanomaterials；tissue repair；induction of differentiation；cell translation