基于UV-B信号转导途径的梨果皮花青苷合成光信号应答分子机制解析

项目名称： 基于UV-B信号转导途径的梨果皮花青苷合成光信号应答分子机制解析

项目编号： No.31301753

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 张东

作者单位： 西北农林科技大学

项目金额： 22万元

中文摘要： 光信号可作用于植物光受体，并通过信号转导途径逐级向下游传递信号调控花青苷合成。红梨花青苷合成需要高强度的光照，目前对受光调控下游直接参与花青苷合成的结构和调节基因的关注较多，忽略对上游间接调控花青苷合成的光信号转导途径基因的研究，此外，试验设计中，没有细分不同光质，没有选用具有不同光应答效应的试材，难以全面认识梨花青苷合成光信号应答分子机制。本研究，拟以具有不同光应答效应红色砂梨和西洋梨典型品种为试材，通过控制UV-B和可见光照射条件，诱导红梨花青苷差异积累，筛选关键UV-B信号转导基因，利用凝胶迁移、酵母双杂交和双分子荧光互补等技术分析候选基因与花青苷合成结构和调节基因在体内外的互作调控关系，再利用拟南芥转基因和梨果实瞬时表达系统鉴定其功能。研究成果有助于阐明梨花青苷合成的UV-B信号应答分子机制，进一步丰富果实花青苷合成的生物学理论，同时对于指导优质红梨生产的着色调控具有现实意义。

中文关键词： 红梨；花青苷；光照；信号转导；调控机制

英文摘要： Plant photoreceptors receive light signal, and transfer the signal to the biosynthesis of downstream procedures including anthocyanin, step by step. Red pears belonging to different species with different genetic backgrounds and coloration patterns may have the distinct transcriptional regulation for related anthocyanin biosynthesis genes compared with apple. Anthocyanin biosynthesis in red pears is a high light involved process. More attention was paid to the downstream structural and regulatory genes directly related to anthocyanin synthesis in the study of molecular mechanism of coloration in red pears, while upstream genes indirectly involved in UV-B signal transductionhave been ignored. What's more, the limited and single materials and light treatments in the previous studies related to red pear would not be favorable to the comprehensive understanding of molecular regulation mechanism of different kinds of red pears with different abilities in response to light signal. Therefore, in this project, typical red pears including Oriental pear and Occidental pear with different coloration patterns will be used to study molecular mechanism of coloration in red pears in response to light signal. Different light treatments will be used to induce different accumulation of anthocyanin in different red pears. Key cand

英文关键词： Red pears；anthocyanin；light；signal transduction；regulatory mechanism