蓖麻矮化相关RcDof基因功能分析及调控机制研究

项目名称： 蓖麻矮化相关RcDof基因功能分析及调控机制研究

项目编号： No.31460353

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 陈永胜

作者单位： 内蒙古民族大学

项目金额： 51万元

中文摘要： 蓖麻是内蒙古地区具有区域特色的油料作物，矮秆蓖麻品种具有单产较高、适合密植和机械化生产等优点。蓖麻矮化育种研究开展多年，但矮秆基因的转录调控机制及作用机理尚不清楚。Dof(DNA-binding with one finger)蛋白是植物特有的一类转录因子，在植物生长发育过程中起着重要的作用，可以调控植株矮化。本课题组在以往的研究中发现了一个锌指蛋白RcDof基因，该基因在矮秆蓖麻材料中表达下调。因此，本研究拟克隆蓖麻锌指蛋白RcDof基因，转化高秆蓖麻材料，分析该基因的功能；明确RcDof基因作用的靶基因、调节途径及与RcDof基因编码蛋白相互作用的蛋白质，探讨其矮化调节机制,为蓖麻矮化育种提供新材料，为提高蓖麻单产奠定基础。

中文关键词： 蓖麻；矮化；RcDof基因；功能分析；调控机制

英文摘要： Castor (Ricinus communis) is a typical oilseed crop which is widely cultivated in Inner Mongolia. The dwarf castor has many advantages, such as high yield, high density planting and mechanization, etc. Although breeding in dwarf castor has been studied for many years, the mechanics of transcription in dwarf gene is unclear. The Dof (DNA-binding with one finger)domain proteins are plant-specific transcription factors with highly conserved DNA-binding domain, and play critical roles as transcriptional regulators in plant growth and development, including regulating plant dwarfing. In our research, a Dof gene (RcDof gene) of zinc finger protein was found that involved in the process of castor dwarfing. The purpose of this study is to clone RcDof gene, transform it into high stalk castor and analyse its function. According to determining the target genes, regulating pathways and protein-protein interaction, we can study its regulatory mechnism of dwarf. Our research will provide new germ plasma resources, and contribute to improving the yield of castor.

英文关键词： Castor;Dwarf;RcDof gene;Functional analysis;Regulatory mechanism