基于分子印迹聚离子液体水凝胶阵列的肿瘤标志蛋白电化学传感研究

项目名称： 基于分子印迹聚离子液体水凝胶阵列的肿瘤标志蛋白电化学传感研究

项目编号： No.21275166

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 李春涯

作者单位： 中南民族大学

项目金额： 85万元

中文摘要： 项目旨在以聚离子液体水凝胶纳米阵列制备分子印迹聚合物，在水溶液中构建对模板蛋白质具有特异识别能力的电化学传感器，建立快速、高选择性、高灵敏度的肿瘤标志蛋白电化学分析新方法。拟采用新型离子液体类功能单体，经适度交联，借助聚碳酸酯阵列模板和电化学调控技术，水相制备对目标蛋白质具有高选择性识别的聚离子液体水凝胶纳米阵列；利用离子液体的水溶性、生物相容性和丰富的、可用于分子识别的官能团，以保持目标蛋白的空间结构的稳定性和多种弱相互作用力，结合纳米阵列结构提高印迹因子和传质速率；依托电极的刚性表面维持水凝胶稳定性，使印迹水凝胶阵列保持最佳的识别位点。系统考察识别特性参数与制备参数之间的内在规律，获取分子印迹阵列传感器的识别机理和制备新原理和新技术；借助电活性探针/纳米信号放大技术，研究肿瘤标志蛋白的信号转换机制、传感特性参数、敏感规律，建立电化学传感新理论及痕量快速检测新方法，并用于实际生物样品分析

中文关键词： 离子液体；分子印迹；肿瘤标志蛋白；纳米增敏；电化学传感

英文摘要： The objective of this proposal is to prepare molecularly imprinted polymers using poly(ionic liquid) gels and fabricate different nano-array electrochemical sensors with specific recognition ability towards certain template protein (such as tumor markers) in aqueous solution. Based on these new nano-array sensors, electroanalytical methods for the determination of tumor markers with high specificity and sensitivity at low concentration and fast speed under simple conditions can be developed. The new molecularly imprinted nano-array gel is prepared with electrochemical techniques in aqueous solution using an ionic liquid as a functional monomer and a porous polycarbonate film as an array template with a suitable amount of cross-linking agent. The imprinted ratio and mass trasfer rate can be improved based on the control of the structure of nano-array gel, the solubility, the biocompatibility, and the functional groups of ionic liquids which are able to enhance the conformation stability and the interaction between the function monor and the template protein. The weak mechanical strength of poly(ionic liquid) gel is overcomed by using a rigid electrode surface, the sensing surface modified with imprinted polymers is thus able to maintain the most suitable imprinted sites for protein recognition. The relationship b

英文关键词： Ionic liquid；molecular imprinting；tumor marker；nano-size in enhancing effect；electrochemical sensing