PBD-1修饰的BMSCs移植治疗猪支原体肺炎的实验性研究

项目名称： PBD-1修饰的BMSCs移植治疗猪支原体肺炎的实验性研究

项目编号： No.31201938

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 畜牧学与草地科学、兽医学、水产学

项目作者： 黄海军

作者单位： 武汉市畜牧兽医科学研究所

项目金额： 23万元

中文摘要： 研究和开发一种新的无药物残留、无耐药性的净化猪支原体肺炎的有效途径对于养猪业的健康发展具有重要的科学意义和实用价值。本项目是基于目前骨髓间充质干细胞体内移植的最新理论和临床研究基础，结合前期疫苗研发过程中发现转PBD-1细胞培养上清液对肺炎支原体有较好抑制效果的现象，创新性地运用转染PBD-1的猪自体骨髓间充质干细胞移植到感染猪肺炎支原体的试验猪体内，探讨PBD-1净化支原体感染的效果并分析相关免疫学效应。本项目研究获得的成果将不仅为猪支原体肺炎的治疗提供一条崭新的有效途径，也为其他抗生素耐药性动物疾病甚至是某些人类疾病的治疗提供相关科学依据。

中文关键词： 猪支原体肺炎；骨髓间充质干细胞；干细胞治疗；遗传修饰；动物模型

英文摘要： To make research and develop a new approach of treating Swine Mycoplasma hyopneumoniae without drug residue and resistance exerts an important influence as well as utility value on the healthy development of swine husbandry. The project is implemented on the basis of the newest theory and experimental study of genetic-modified Bone Mesenchymal Stem Cell, combing with the phenomenon of comparatively positive effect the culture supernatant exerted on Swine Mycoplasma hyopneumoniae in the previous vaccine development,with the innovative application of implanting Bone Mesenchymal Stem Cells genetic-modified with PBD-1 into the experimental swine infected by Swine Mycoplasma hyopneumoniae，to explore the effective of purifying Swine Mycoplasma hyopneumoniae infection, and study the immune effect of PBD-1. The result of the study will have not only provided a brand new effective approach to curbing Swine Mycoplasma hyopneumoniae,but also the scientific reference for the treatment of antibacterial and drug-resistant animal disease and even some human disease.

英文关键词： Pneumonia of Swine；Bone Mesenchymal Stem Cell；Stem cell therapy；Genetic-modified；Animal model