电针"足三里"对脊髓损伤结肠动力障碍大鼠per2基因生物钟效应的研究

项目名称： 电针"足三里"对脊髓损伤结肠动力障碍大鼠per2基因生物钟效应的研究

项目编号： No.81202735

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 朱毅

作者单位： 南京中医药大学

项目金额： 23万元

中文摘要： 用重物坠落法(WD)造成脊髓损伤大鼠模型，以结肠基本电节律、收缩复合机电为指标了解脊髓损伤后大鼠结肠功能状态。采用免疫荧光标记、免疫组化双染色技术、实时荧光聚合酶链反应等技术，观察脊髓损伤后肠功能障碍大鼠per2周期性表达的变化、nNOS及ACh周期性表达变化，三者与结肠周期性节律性收缩、BER的关系；观察电针足三里对脊髓损伤大鼠per2基因和nNOS、ACh两种重要神经递质周期性表达的影响，并与全胃肠动力药莫沙必利比较。为电针足三里调节脊髓损伤后肠功能障碍，为针灸临床开展针灸对胃肠道生物钟调节作用提供理论和实验依据。

中文关键词： 电针；脊髓损伤；结肠；per2基因；生物钟节律

英文摘要： Many spinal cord injury (SCI) patients suffer from disfunction of gastric empty and purging the bowels which affect their physical or psychological health and even life-span. Fortunately, electro-acupuncture has been accepted to manage the disfunction of gastrointestinal tract. In addition, it is economical, safe and convenience for patients. However, its mechanism and the accurate tache that electro-acupuncture works are still unclear. Our study concentrate on the bowel movement and colonic slow waves by observation fecal matters, basic electrical rhythm and contratile electrical complex, while learn the rhythmic change in per2mRNA, nNOSmRNA and nNOS of colonic longitudinal muscle myenteric plexus by immunohematology, Immuno-histochemistry double staining method and quantitative real time polymerase chain reaction method before and after electro-acupuncture and Mosapride . So that we can observe their relationship above, estimate the effect after acpuncture and Mosapride and learn the possible mechanism. That will enrich the basic theory of acupuncture modulating the disfunction of gastricintestinal tract and time therapy of acupuncture, provide the evidence of pathogenesis and therapeutic mechanism of disfunction of gastrointestinal tract after spinal cord injury. What's important, it will provide a better cho

英文关键词： electroacupuncture；spinal cord injury；colon；period2 gene；circadian cycle