桃红肉位点DBF和bf的精细定位和关键基因解析

项目名称： 桃红肉位点DBF和bf的精细定位和关键基因解析

项目编号： No.31471848

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 俞明亮

作者单位： 江苏省农业科学院

项目金额： 85万元

中文摘要： 水果的营养与保健价值越来越受关注，一些红肉桃果实抗氧化能力可与蓝莓相媲美，红肉性状既能迎合消费需求又能丰富桃果类型，成为研究热点。现有研究已证实桃红肉性状存在显性遗传（DBF）和隐性遗传（bf）两种类型。项目前期研究已分别将DBF和bf初定位于第5和第4连锁群，生物信息学预测桃红肉的分子机制可能完全不同于其他蔷薇科果树。本项目拟开展的主要研究：⑴利用大群体和多群体，分别完成DBF和bf的精细定位；⑵通过亲本及典型资源的全基因组重测序，搜索精细定位区段内的差异位点，并在种质资源群体中发掘与红肉性状相关的位点；(3)通过转录组分析和RT-qPCR验证，锁定DBF和bf关键基因或调控元件；(4)设计共显性的MAS标记，用于种质资源的基因型鉴定和红肉桃分子育种。本项目的实施将能：揭示红肉桃花色苷代谢的分子机制；将MAS技术首次应用于红肉桃育种；为红肉桃种质资源挖掘与创新利用提供理论支撑。

中文关键词： 红肉桃；显性红肉基因；隐性红肉基因；精细定位；分子机制

英文摘要： Nutrition and health benefits contained in daily fruit were highlighted by consumers. Some blood-flesh peach accessions were identified to have very high antioxidant capacity which is comparable to blueberry. Due to its health benefits and attractive colour, the blood-flesh traits are emphasized in peach research nowadays. Two types of blood-flesh traits, dominant blood-flesh (DBF) and recessive blood-flesh (bf), were found in peach genetic resources. In previous study, we mapped DBF and bf loci on linkage 5 and linkage 4, respectively. Bioinformatics analysis in the DBF and bf region predicted a novel mechanism underlining blood-flesh traits comparing to other Rosaceae family. This program was designed (1) to fine map DBF and bf loci by using large and multiple populations, respectively; (2) to search polymorphic loci between blood genotypes and non-blood ones in the mapping regions by whole genome re-sequencing of the parents and typical blood-flesh accessions; (3) to discover the key genes or regulators controlling blood-flesh by transcriptome analysis and RT-qPCR analysis; (4) to design co-dominant markers for marker assisted selection (MAS), and apply them in identifying germplasm collections and in breeding practice. By running the project, the molecular mechanism underlying the anthocyanin accumulation in each blood-flesh genotypes will be discovered, effective markers for MAS will be developed, and theoretical informations supporting utilization and innovation of blood-flesh peach germplasm will be obtained.

英文关键词： blood-flesh peach;dominant blood-flesh (DBF);blood-flesh (bf);fine mapping;molecular mechanism