大黄鱼早期发育过程对甲基汞胁迫的响应及其机制研究

项目名称： 大黄鱼早期发育过程对甲基汞胁迫的响应及其机制研究

项目编号： No.41306112

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 天文学、地球科学

项目作者： 黄伟

作者单位： 国家海洋局第二海洋研究所

项目金额： 25万元

中文摘要： 甲基汞(MeHg)是汞在海洋生物体内的主要赋存形态，对海洋生物神经系统、内分泌系统具有极强毒性，但迄今甲基汞对海水鱼类早期发育过程毒理效应及机制的研究十分薄弱。本项目选取大黄鱼作为研究对象，综合运用代谢组学和生物化学方法系统研究甲基汞对其胚胎和仔稚鱼的毒理效应，阐明甲基汞在大黄鱼各组织中的蓄积动力学特征，解析大黄鱼早期发育过程抗氧化防御系统和内源性代谢物对甲基汞胁迫的响应方式。项目探索性地将代谢组学技术应用于污染物对海水鱼类早期生活阶段毒理效应的研究中，旨在从代谢水平揭示甲基汞对海水鱼类早期生活阶段的致毒机制。本研究可为代谢组学技术在生态毒理学中的应用提供研究实例，同时为科学认识近海污染胁迫下海洋生物资源的衰退机制提供理论依据。

中文关键词： 大黄鱼；胚胎；仔鱼；甲基汞；代谢组学

英文摘要： Mercury is one of the most toxic metals in marine environments. It causes various damages to the central nervous system and endocrine system of fish as well as disrupt vital functions such as reproduction, osmoregulation, orientation, foraging, predator recognition and communication in fish. Mercurials could be biologically transformed into more toxic and bioavailable organic form-methylmercury. However, studies have been more carried out to investigate the toxicity of inorganic mercury than that of organic mercury. Early life stages (ELS) are of primary importance to fish population dynamics due to the intense mortality during the embryonic and larval periods. Fish embryos and larvae are particularly sensitive to natural and anthropogenic origin stressors including mercury exposure. To our knowledge, there is little literature about the responses of ELS of marine fish species to methylmercury, especially about the changes of its metabolic products and antioxidants. In this research program, biochemical and metabolomics techniques are used to investigate the toxicological effects of methylmercury exposure on the development and growth of large yellow croaker embryos and larvae in order to: (1) evaluate the accumulation dynamics of methylmercury in different stages and tissues of embryos and larvae, (2) investiga

英文关键词： Large yellow croaker；embryo；larvae；MeHg；metabolomics