Notch和Wnt信号对鹿茸间充质干细胞自我更新与软骨分化的调控机制研究

项目名称： Notch和Wnt信号对鹿茸间充质干细胞自我更新与软骨分化的调控机制研究

项目编号： No.31260541

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 韩春梅

作者单位： 塔里木大学

项目金额： 51万元

中文摘要： 鹿茸间充质干细胞是一类新的干细胞，是除骨髓间充质干细胞外研究成体干细胞体外诱导软骨发生极好的模型。在鹿茸生长发育过程中，它一方面能够不断增殖更新干细胞库，还能够不断向软骨分化，维持鹿茸类似肿瘤的快速生长却不发生癌变，这种特性与微环境中Wnt 和Notch信号通路对间充质干细胞的调控密不可分。本研究以新疆塔里木马鹿茸间充质细胞为材料，通过三维细胞培养和诱导，鉴定其干细胞表型特征，为体外培养和诱导软骨生成提供实验依据；利用蛋白印迹法、实时定量PCR及RNAi等技术，研究Notch信号对间充质细胞向软骨分化的作用，初步揭示该信号对软骨分化作用机制；在上一个基金对c-myc基因的研究基础上，研究Notch和Wnt信号在诱导软骨发生过程中的相互作用关系，揭示鹿茸间充质细胞增殖分化自我平衡的分子机制，为鹿茸快速生长机制提供理论支持，同时为以鹿茸间充质细胞诱导骨生成作为人类骨病治疗提供实验材料和线索。

中文关键词： 马鹿茸间充质干细胞(MSCs)；Wnt信号；Notch信号；软骨分化；增殖

英文摘要： Deer antler mesenchymal stem cells(MSC) are a newly discovered stem cell that is unique to deer. And it provides an excellent model to study microenvironmental effects on somatic stem cell proliferation and chondrocytes differentiation except bone marrow mesenchymal stem cells. In the process of deer antler growth the antler MSC differentiate into chondrocytes constantly to promote antler growth, however the MSC also continue to proliferate in order to maintain the pool of stem cells. Regulation of MSC proliferation and differentiation by the Wnt and Notch signaling pathways is responsible for the antler's characteristic rapid growth but somehow avoids the formation of cancer which would be expected in other tissues dividing so rapidly. In my study Xinjiang Wappti antler MSC will be 3D cultured and induced in vitro. Its cells phenotype will be identified by Flow Cytometry. The result could be an experiment supportor for inducing deer antler MSC differentiation into chondrocytes in vitro . Methods such as westernblot, realtime PCR and RNAi could then be used to study the effect of Notch and Wnt signaling pathways on chondrocytes differentiation from antler MSC,and the functional mechanism of Notch signaling pathway and the molecular mechanism that homeostasis of MSC proliferation and differentiation will be eluci

英文关键词： Deer antler’s mesenchymal stem cells(MSCs)；Wnt signaling pathways；Notch signaling pathways；differentiation；proliferation