一个水稻组织特异性脆秆突变体hk06的基因克隆与功能解析

项目名称： 一个水稻组织特异性脆秆突变体hk06的基因克隆与功能解析

项目编号： No.31301297

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 刘斌美

作者单位： 中国科学院合肥物质科学研究院

项目金额： 23万元

中文摘要： 水稻茎秆的机械强度是一个重要的农艺性状，在水稻的抗倒性、产量以及秸秆利用等方面起着重要作用，而茎杆的机械强度主要是由细胞壁提供。目前关于水稻细胞壁的生物合成和调控机理并不完全清晰，需要发掘和解析更多的新基因。本项目拟以一个重离子诱变的组织特异性脆秆水稻突变体hk06为研究对象，利用细胞学和化学分析手段鉴定突变体在细胞结构和组织成分上的差异；在前期定位研究的基础上，进一步精细定位、筛选和确定控制hk06脆秆性状基因 tsbc1(t)的候选基因；转基因互补进行功能验证；分析tsbc1(t)基因的编码蛋白，并预测其可能的功能；研究 tsbc1(t)基因在不同发育时期与部位的表达特性，初步解析新基因的功能以及在水稻细胞壁生物合成中所处的环节。通过对tsbc1(t)基因克隆和功能解析，有助于揭示水稻细胞壁生物合成和机械强度形成的分子机制，为从品种源头上解决水稻秸秆还田难题提供新的研究思路和理论基础。

中文关键词： 水稻；脆秆；cef1；细胞壁；转录因子

英文摘要： Mechanical strength is an important agronomy trait of rice plants that affects crop lodging, grain yield and straw utilization. As a prominent physical property of cell walls, mechanical strength reflects upon the structure of different wall polymers and how they interact. Studies on the mechanisms that regulate the mechanical strength therefore consequently results in uncovering the genes functioning in cell wall biosynthesis and remodeling. Several brittle mutants of rice were identified and characterized. In this study, we characterized a new brittle mutant hk06 identified from japonica variety xiushui110 by means of ion beam radiation. To understand the mechanism of controlling plant mechanical strength, the anatomical structure and chemical features of brittle mutant hk06 were investigated. Anatomical analyses were carried out by means of various microscopic techniques, such as light microscopy, scanning electron microscopy and transmission electron microscopy. Based on the preliminary gene mapping, the candidate gene of tsbc1(t) will be isolated by chromosome walking. We plan to take the transgenic complementary technology to verify the accuracy of the identified gene. Then, the gene structure and predicated functions of protein will be speculated through bioinformatics analysis method. In the other hand,

英文关键词： rice；brittle culm；cef1；cell wall；transcription factor