云南边境口岸艾滋病病毒跨境传播特性与演化规律研究

项目名称： 云南边境口岸艾滋病病毒跨境传播特性与演化规律研究

项目编号： No.81460509

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 夏雪山

作者单位： 昆明理工大学

项目金额： 55万元

中文摘要： 艾滋病病原体--人类免疫缺陷病毒（HIV）高度变异、频发重组，根据基因序列同源性可将其分为多种基因亚型/重组亚型。云南省地处我国西南边境，与东南亚多国接壤，是HIV-1传入我国并进一步重组的主要地区，对我国HIV-1病毒跨境传播特性、病毒重组规律及其演化规律的研究十分重要。本项目拟完成云南17个口岸12年来出入境人员HIV-1阳性血浆样本（2700余份）的储存及相应信息收集的基础上，继续对该人群进行艾滋病感染情况监测；通过HIV-1阳性样本病毒基因扩增、序列测定与分析，阐明该人群中HIV-1基因亚型、重组亚型分布特性；对主要亚型/重组亚型进行进化分析，阐明其历史演化规律。本研究对象具有采样地域代表强、样本数量大、时间跨度长的特点，可系统地反映云南省HIV-1跨境传播特性和演化规律。预期成果可监测HIV-1跨境传播特性、阐明其历史演化规律、并预测其流行趋势，为我国艾滋病控制提供流行病学依据。

中文关键词： 艾滋病；口岸；基因型；跨境传播；演化

英文摘要： The pathogen of Acquired Immunodeficiency Syndrome (AIDS), Human Immunodeficiency Virus (HIV) was characterized as high mutation and frequent recombinants. According to their phylogenetic relationship, HIV could be classified into various genotypes and recombinants. Yunnan, the most south-eastern province in China, was neighbored with Southeast Asian countries. The specific geographic location made it become the first station of HIV-1 spreading into China, and the hotspot where HIV-1 recombinants occurring. Yunnan has always played the most important role in the research of HIV-1 cross-border transmission and its historical evolution. In regard to this, based on the samples collected from HIV-1 positive cross-border travelers of all the 17 entry ports in Yunnan since 2002, we are planning to continue the monitoring on HIV-1 infection among cross-border travelers for further understanding of HIV cross-border transmission. To clarify the HIV genotypes and recombinants distribution among cross-border travelers and their evolution characters, the stored and newly collected samples were conducted with target gene amplification, sequencing and followed analysis. This research was characterized as geographic specific, the long period of sample collecting. HIV cross-border transmission characters and its evolution could be well reflected. It is also possible to discover the novel genotype/recombinant. Our findings could provide the epidemiological data for the monitoring of HIV cross-border transmission, clarifying its evolution and forecasting of HIV spread.

英文关键词： AIDS;ports;genotype;cross-border transmission;evolution