玉米株型相关的耐旱基因/QTL定位及位点互作效应研究

项目名称： 玉米株型相关的耐旱基因/QTL定位及位点互作效应研究

项目编号： No.31301333

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 彭云玲

作者单位： 甘肃农业大学

项目金额： 21万元

中文摘要： 玉米的株型性状与耐旱性关系密切，紧凑株型玉米与平展株型相比有较强的耐旱性。因此研究紧凑株型玉米的耐旱机理，定位株型相关的耐旱基因/QTL，对培育紧凑株型玉米新品种、减少干旱造成的损失具有重要意义。本研究以玉米自交系廊黄和昌7-2分别与TS141组配构建的两个F2:3群体为材料，采用逐步联合的QTL定位方法，在对干旱胁迫最敏感的大喇叭口期检测株型相关（叶夹角、叶向值、叶形值）的耐旱QTL。将QTL信息整合，获得株型相关的耐旱QTL的一致性区间(MQTL)；同时预测MQTL所在染色体区段的候选基因。构建近等基因系研究株型相关的耐旱MQTL位点之间的互作；利用精细定位群体对控制玉米株型相关的耐旱QTLs精细定位。这些结果将为候选基因的克隆和紧凑株型玉米耐旱性分子机理的研究提供理论基础。

中文关键词： 玉米；株型性状；耐旱性；元分析；候选基因

英文摘要： Maize architecture traits are closely related to drought tolerance. Compared with flat plant type maize, compact plant type maize has stronger drought tolerance. So, it is of great significance to study drought tolerance mechanism of compact plant type maize and to map plant-type traits related gene/QTL for cultivating compact plant-type maize varieties and reducing drought loss. In this study two F2:3 populations derived from Langhuang×TS141 and Chang7-2× TS141 will be used to detect drought tolerant QTLs related to maize architecture traits(such as leaf angle, leaf orientation value, leaf shape value) under different water regimes in the bell-mouthed period when maize is most sensitive to drought tolerance in Wuwei Gansu province by stepwise joint QTL mapping method. These QTLs in this study will be integrated into informations of the published QTLs relevant to maize plant type, and drought tolerant MQTL related to plant type will be mapped through the Meta-QTL analysis; at the same time, candidate genes relevant to maize plant type will be speculated which are located on MQTL regions through comparative genomics and bioinformatics. Then, we will study interaction effect between MQTLs sites controlling drought tolerance related to maize architecture traits by the means of building near isogenic lines. Combinin

英文关键词： Maize (Zea mays L.)；Plant architecture trait；Drought tolerance；Meta-analysis；Candidate gene