流行性腮腺炎S79株疫苗抗原交叉性与腮腺炎病毒基因演变的分子流行病学研究

项目名称： 流行性腮腺炎S79株疫苗抗原交叉性与腮腺炎病毒基因演变的分子流行病学研究

项目编号： No.81502860

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 刘元宝

作者单位： 江苏省疾病预防控制中心

项目金额： 18万元

中文摘要： 流行性腮腺炎是由腮腺炎病毒引起的急性呼吸道传染病，可引起儿童脑膜炎、胰腺炎，多在幼儿园、中小学暴发流行。当前腮腺炎疫苗免疫策略下疫情依然高发的态势使其成为严重的公共卫生问题。关于S79株疫苗对我国流行的腮腺炎F基因型野毒株的抗原交叉能力与腮腺炎基因演变的研究甚少。本研究通过腮腺炎高发人群血清流行病学调查，利用嵌套混合分布模型，建立疫苗接种人群不同IgG抗体水平分组的前瞻性队列，观察各个抗体水平组腮腺炎发病率，结合交叉中和试验，研究疫苗接种后不同抗体水平与发病关系，探索疫苗接种人群抗体水平衰减规律；结合腮腺炎病毒基因序列研究，探讨SH、HN、F、NP基因片段的人群变异分布，研究腮腺炎病毒基因氨基酸区域位点变异与S79株疫苗交叉中和能力降低相互关系。项目的完成将进一步丰富我国腮腺炎病毒库和基因型别数据库，并为腮腺炎疫苗免疫策略调整，S79株疫苗株筛选优化以及新腮腺炎疫苗毒株开发等提供科学依据。

中文关键词： 流行性腮腺炎；疫苗接种；基因变异；氨基酸位点；混合分布

英文摘要： Mumps is an acute respiratory infection caused by the mumps virus. Mumps virus mainly affects the parotid gland of complications of pancreatitis, meningoencephalitis, with high proportion of outbreaks occurring in kindergarten, primary and secondary school. The high incidence under the strategy of MMR has lead it to the secious public health. Crossprotection of S79 vaccine strain to F genotype of wild strains and evolution of mumps gene was scarce. In this study, we conduct the serological surveys of mumps antibodies in the children with high incidence and surveillance of mumps vrisus collection. Through the mixture distribution model fitting multi-level distribution of IgG antibody populations and cross-neutralization test, we build cohort to seek a protective human antibody levels and attenuation effects evolution trend; and the mumps virus F,G genome sequencing, focusing on analysis SH, HN, F, P genetic variation in the population, are combined to find the mutation sites, the complements of this study will integrate the above information to give assessment of mumps vaccine immune protection and durability, which will further enrich wild mumps virus database and that at the same genotype for the development and adjustment of mumps vaccine strategy, S79 strain screening and optimization of new mumps vaccine strains to provide basic reference vaccine strains of development.

英文关键词： mumps;immunization;Gene mutation;Amino acid position;mixture model