聚多巴胺辅助羟基磷灰石纳米涂层复合骨髓间充质干细胞实现人工角膜生物整合的研究

项目名称： 聚多巴胺辅助羟基磷灰石纳米涂层复合骨髓间充质干细胞实现人工角膜生物整合的研究

项目编号： No.31271059

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 王丽强

作者单位： 中国人民解放军总医院

项目金额： 80万元

中文摘要： 各种原因导致的严重眼表损伤及角膜盲，传统角膜移植成功率很低,合成的硬质人工角膜是这类患者脱盲的希望。目前影响人工角膜发展的主要问题是其和周围组织不能生物整合，可造成严重并发症。本课题拟采用HAp纳米涂层对人工角膜材料表面改性，增加角膜细胞和细胞外基质在杂化界面的粘附、增殖，并减轻炎症反应。并拟使用BMSCs体外诱导软骨细胞，复合在涂层表面，观察角膜细胞因子和细胞外基质与干细胞的相互作用；通过动物实验动态观察植入HAp涂层人工角膜以及复合BMSCs后与周围组织的安全性和有效性；探讨其植入的可行性；通过检测细胞外基质及金属蛋白酶的表达，观察植入术后角膜周围微环境的变化；应用基于OCT气冲印压系统，比较涂层及BMSCs复合后角膜生物力学改变，以及力学刺激对人工角膜周围增殖组织蛋白的表达与调控，评价其在体效果，为解决人工角膜长期稳定性的问题提供实验基础，促进人工角膜技术的发展。

中文关键词： 人工角膜；纳米涂层；生物整合；生物力学；骨髓间充质干细胞

英文摘要： The cornea is the first line of defense for the optical system. Any material that replaces it must integrate with the surrounding tissue such that the interface provides a bacteria tight seal and a mechanically stable attachment. This is where most attempts to replace corneal function have failed. PMMA has remained a popular material of choice in KPro because of its excellent optical properties and biocompatibility, although its biointegration in cornea tissue is known to be poor. Here, polydopamine coating, which has demonstrated excellent adhesive properties, and subsequent modification with Hydroxyapatite (HAp) was applied to the PMMA surface with the prospect that it may enhance the integration of the material with the corneal tissue. To assess the feasibility of polydopamine coating in enhancing the integration with the corneal tissue, previous in vitro studies were performed using both corneal epithelial cells and the keratocytes. Polydopamine coating and subsequent modification with HAp significantly enhanced cellular proliferation of both cell types. Because of the host cornea's insufficiency and weekly proliferative capacity, autologous bone mesenchymal stem cells (BMSCs) will be combined with HAp coated PMMA surface. General cornea response to the polydopamine-HAp coating or/and combined with BMSCs in

英文关键词： keratoprosthesis；nano-coating；biological integration；biomechanics；mesenchymal stem cells