功能化石墨烯分子印迹手性SPR传感器对手性氨基酸的识别及其机理研究

项目名称： 功能化石墨烯分子印迹手性SPR传感器对手性氨基酸的识别及其机理研究

项目编号： No.31501554

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 徐小艳

作者单位： 华南农业大学

项目金额： 21万元

中文摘要： 食品中D-氨基酸的含量是反映食品组成、生物功能及安全性的重要指标。发展快捷、灵敏、实时和在线的手性传感器识别手性氨基酸对于食品营养分析和质量控制十分必要。本项目拟以L-色氨酸为模板，多巴胺为功能单体和交联剂，制备分子印迹聚合物，通过聚多巴胺功能化的石墨烯纳米材料增敏，构筑具有手性识别功能的分子印迹SPR传感器，建立手性氨基酸的快速灵敏准确的识别方法。在实时研究传感膜与色氨酸L-和D-型异构体相互作用过程屮识别动力学和热力学差异的基础上，结合计算机分子模拟技术，分析总结影响手性识别选择性和灵敏度的因素，从分子水平上阐释手性识别机理。. 本项目的完成，将为手性氨基酸的识别分离提供新技术，为系统分析和规避消费者饮食奠定基础；对于食品、药品和环境中等诸多小分子手性物质的识别及其识别机制研究具有普遍的借鉴意义，对于丰富和推进手性识别的理论和实践具有重要的科学意义。

中文关键词： 手性识别；分子印迹；手性传感器；信号放大

英文摘要： The content of D-amino acids (AAs) in foodstuff is an important indicator of food composition, biological function and food safety. It’s necessary to develop a rapid, sensitive, real-time and online AAs chiral sensor for nutrition analysis and quality control of food. This project aims to construct a chiral surface plasmon resonance (SPR) sensor based on functionalized grapheme-molecularly imprinted polymers composite . The composite is synthesized using L-tryptophan(L-Trp) as template molecule, dopamine as functional monomer and crosslinker, graphene as signal amplification agent functionalized by polydopamine. Then a rapid, sensitive and accurate recognition method for chiral AAs can be achieved. On the basis of real-time investigating recognition differences of kinetics and thermodynamic between sensing film and Trp enantiomers by the chiral sensor, computer-aided molecular simulation technology is employed to analyze the chiral recognition factors affecting selectivity and sensitivity. Then the chiral recognition mechanism is explained from the molecular level.. The completeness of this project will provide a new separation technology for chiral AAs and lay the foundation for systematical analysis and avoiding contaminated diet for consumers. Meanwhile, this work has general reference meaning for small chiral molecular in food, medicine and environment, great scientific significance for enriching and promoting the theory and practice of chiral recognition.

英文关键词： Chiral recognition ;Molecularly imprinting;Chiral sensor ;Signal amplification