非人类灵长类动物（猕猴）脊髓撞击损伤模型的建立和行为学评估

项目名称： 非人类灵长类动物（猕猴）脊髓撞击损伤模型的建立和行为学评估

项目编号： No.81200940

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 神经系统疾病、精神疾病

项目作者： 马政文

作者单位： 上海交通大学

项目金额： 23万元

中文摘要： 脊髓损伤（SCI）是机体遭遇的最具破坏性的外伤之一，其致残的后果给患者、家庭和社会带来严重负担。虽然实验室研究取得了一些成果，但向临床转化过程中遇到了困难。原因之一是实验室研究对象大多是啮齿类动物，其解剖结构、生理功能等与人类有很大的差异。而灵长类动物与人类十分接近，可以作为实验室成果向临床转化的中间平台。本研究将以猕猴作为研究对象，首次使用针对灵长类动物的专用重物坠落损伤仪，建立猕猴脊髓撞击伤模型，更好地模拟临床SCI情况。另外，灵长类动物脊髓损伤前后行为学及功能鉴定，在国际上并没有的统一标准，为准确评价非人灵长类SCI模型的稳定性、各种治疗措施的有效性等重要指标增加了难度。因此，本项研究我们将建立一系列有针对性、敏感的灵长类动物脊髓损伤行为学评估，并首次使用Hargreaves方法检测猕猴痛温觉反射。本课题将为国际上灵长类动物脊髓损伤行为学评价体系的完善和统一提供宝贵的资料。

中文关键词： 脊髓损伤；非人灵长类(猴)；行为学评估；电生理；Hargreave's

英文摘要： Spinal cord injury (SCI) is one of the catastrophic diseases that significantly affect the quality of life of the patients, and huge economic burden to their family and the entire society. Progresses on SCI research have been made in recent years,however, there is no effective treatment available in the clinic yet. One reason for the lack of effective treatment may be due to the fact that commonly used rodent models are less translational to the clinic. There are significant differences in anatomical structures and pathophysiolocial properties between rodents and human patients. Using non-human primates in the SCI research should bridge the rodent studies to humans, which is highly clinic relevant. However, to date, limited SCI studies have been done in nonhuman primates. In this application, we propose to develop a well-controlled, highly-reproducible spinal cord impact model in rhesus monkeys to establish a baseline for investigating injury mechanisms, and assessing recovery of function after SCI. The model is characterized by a firm veterbral stabilizaiton and accurate graded tissue displacements. When established, this model can be used to assess novel therapeutic interventions for patients with SCIs. Our SCI model on nonhuman primates will generate reproduce cord lesion to simulate the clinical situation of

英文关键词： spinal cord injury; SCI；non-human primate(monkey)；behavior assessments；electrophysiology；Hargreave's