基于金纳米颗粒局域表面等离子体共振的暗场散射成像传感分析

项目名称： 基于金纳米颗粒局域表面等离子体共振的暗场散射成像传感分析

项目编号： No.21275011

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 李娜

作者单位： 北京大学

项目金额： 80万元

中文摘要： 金属纳米材料局域表面等离子体共振（LSPR）为光学传感提供了高灵敏、信号可调、易与识别单元偶合的信号转换单元。基于金属纳米材料LSPR光散射的暗场成像设备简单，具有良好的空间分辨功能，在生物传感、细胞成像以及医学诊断方面具有明显优势，成为金属纳米材料LSPR光学传感的重要发展方向之一。本项目拟围绕金纳米材料LSPR光学传感理论与应用这一科学问题，开展基于金纳米颗粒LSPR光散射的暗场成像传感方法研究。通过探讨不同形状、结构（尤其是DNA介导组装的金纳米晶体和硅胶@金核壳型这两类复合结构）的金纳米颗粒LSPR光散射性质在生物传感识别过程中的变化规律，丰富和发展金纳米材料LSPR光学传感的理论与应用；以生物大分子和细胞为传感对象，探索各种识别模式与金纳米材料偶合的传感性能，构建金纳米颗粒LSPR光散射暗场成像传感平台；尝试单纳米颗粒LSPR暗场散射光谱传感，构建高灵敏度、高选择性的传感方法。

中文关键词： 金纳米；暗场散射成像；单颗粒计数；金属增强荧光；核酸

英文摘要： Plasmonic nanomaterials are the type of excellent optical signal transducer because they exhibit high sensitivity,versatility and tunable optical property,and are easy to integrate with biological systems.Localized surface plasmon resonance (LSPR) light scattering property of metal nanomaterials makes it advantageous in sensitive and highly contrasted imaging using very simple but powerful dark-field microscopy,thus plasmonic nanoparticle LSPR light scattering dark-field microscopy has been found promising in biosensing,cell imaging,and clinical diagnosis.This project will focus on developing dark-field microscopic biosensing methods based on gold nanoparticle LSPR light scattering.Other than gold nanoparticles and gold nanorods,the sensing property of composite gold nanomaterials,specifically the DNA-programmable nanoparticle assembly and the core-shell type SiO2@AuNPs,will also be prepared and evaluated to select the suitable nanomaterials with desirable LSPR band and sensitivity for sensing purposes. LSPR light scattering dark-field microscopic method for biomacromolecules and cells will be developed based on highly specific recognition reactions,such as antibody-antigen,functional nucleic acid-target,and saccharide-lectin.Based on results from the above study,the ultimate goal of this project is to establish

英文关键词： Gold nanoparticles；Dark-field light-scattering imaging；Single particle enumeration；metal-enhanced fluorescence；nucleic acids