电纺纳米纤维膜固定化酶及其催化转化典型PPCPs的反应机制

项目名称： 电纺纳米纤维膜固定化酶及其催化转化典型PPCPs的反应机制

项目编号： No.51208368

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 建筑环境与结构工程学科

项目作者： 徐冉

作者单位： 同济大学

项目金额： 25万元

中文摘要： 与传统的纳米颗粒相比，静电纺丝纳米纤维膜有望克服"分离和回收"的难题，降低环境风险，是优良的固定化酶载体。纳米纤维膜固定化酶在环境领域的应用是一个崭新的研究方向。迄今电纺载体材料物理化学性质及其与酶的结合方式对催化转化有机污染物的影响机制尚不明确，对典型环境污染物的催化转化机理研究匮乏。本项目拟针对以上问题开展如下研究：（1）建立以电纺纳米纤维膜为载体的高效固定化酶制备技术，研究材料、载酶形式和酶固定化方法对酶催化动力学特性的影响；（2）以固定化漆酶/辣根过氧化物酶催化转化典型PPCPs-双氯芬酸和三氯生为例，分离和表征转化产物，揭示污染物转化机理；（3）分析固定化酶催化转化PPCPs的主要影响因素，建立反应动力学模型，阐明纳米纤维膜固定化酶催化转化PPCPs的机制。本项目的研究成果将为电纺纳米纤维膜固定化酶在环境领域的实际应用提供新思路和理论依据。

中文关键词： 静电纺丝；纳米纤维膜；固定化酶；污染物；药品及个人护理品

英文摘要： Electrospun nanofibrous membranes (ENMs) have been demonstrated to be excellent carriers for enzyme immobilization since they have a greater chance to overcome the difficulties in "separation and recycle" compared with traditional nanoparticles. Since it is a novel approach to the environmental field, the influence of the physicochemical properties of electrospun carriers and their combination ways with enzymes on catalytic transformation of organic pollutants is still unclear, the research on catalytic transformation mechanisms of typical environmental pollutants is insufficient. The specific objectives of this research and its scope are as follows: (1) to develop the technology of enzyme immobilization on electrospun nanofibrous membranes, study the effect of materials, enzyme-carrying forms and immobilization methods on enzyme catalytic kinetics; (2) to separate and analyze the transformation products of typical pharmaceutical and personal care products, such as diclofenac acid and triclosan, by the immobilized laccase/horseradish, thus reveal the transformation mechanism of the pollutants; (3) to analyze the major impact factors of PPCPs transformation by the immobilized enzymes, establish the reaction kinetics models and summarize the catalytic transformation mechanism of PPCPs by ENMs immobilized enzymes.

英文关键词： Electrospinning；nanofibrous membrane；immobilized enzyme；pollutants；PPCPs