钌配合物功能化聚乙烯亚胺电致化学发光材料的制备及其在免疫传感器中的应用

项目名称： 钌配合物功能化聚乙烯亚胺电致化学发光材料的制备及其在免疫传感器中的应用

项目编号： No.51473136

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 一般工业技术

项目作者： 柴雅琴

作者单位： 西南大学

项目金额： 83万元

中文摘要： 电致化学发光体系中引入共反应剂是提高发光强度的有效途径之一。通常，发光强度的增加是通过发光体与共反应试剂两者分子间的相互作用来实现的，然而，这种方法仍存在发光效率较低的缺陷。针对这一问题，本项目拟设计合成制备多种在同一分子内既含有钌配合物电致化学发光基团又含有胺类共反应基团的新型自增强电致化学发光聚乙烯亚胺（PEI）高分子材料，使其具有电致化学发光效率高、氧化还原可逆性好、易于标记的特性。以肝癌早期诊断具有重要价值的特异标志蛋白（如，AFP、AFU、DCP等）为检测对象，采用钌配合物功能化PEI自增强电致化学发光新材料，构建新型高灵敏电致化学发光免疫传感器。本研究不仅能制备高分子电致化学发光新材料，并将其应用于肝癌特异标志蛋白电致化学发光免疫传感器的构建，同时还为其它蛋白、DNA、生物小分子的检测提供了借鉴和启发，这对拓展高分子材料在生物医学检验及临床疾病诊断的应用具有重要价值。

中文关键词： 电致化学发光；钌配合物功能化聚乙烯亚胺；聚乙烯亚胺；钌配合物；免疫传感器

英文摘要： The introduction of coreactant into chemiluminescence (ECL) system is one of the effective ways to improve the ECL intensity. Generally, the enhancement of ECL signal is based on the molecular interactions between the coreactant and the luminophore, however, this method is still limited by the defect of low luminous efficiency. To solve this problem, this project intends to synthesize the novel functionalized polyethyleneimine (PEI) polymers as electrochemiluminescent material, which contains ruthenium complexes luminophore and amine-rich coreactive groups, possessing the advantages of high electroluminescence efficiency, well oxidation-reduction reversibility and easy labeling. Moreover, ultrasensitive electrochemiluminescence immunosensors based on the self-enhanced ruthenium complexes functionalized polyethyleneimine (PEI) polymers are constructed for the trace determination of eraly liver cancer related serum markers, such as alpha-fetoprotein (AFP), α-L-fucosidase (AFU), des-γ-carboxy-prothrombin (DCP), etc. In conclusion, this project not only realizes the synthesis of novel self-enhanced PEI polymers for the electrochemiluminescence immunosensor, but also provides a reference and inspiration for detecting other proteins, DNA and small molecules with biological active, thus providing a significant value for application of the polymer material in biomedical examination and clinical disease diagnosis.

英文关键词： electrochemiluminescence;ruthenium complexes functionalized polyethyleneimine polymer;polyethyleneimine polymer;ruthenium complexes;immunosensor