石墨烯水凝胶多孔支架修复颅面骨缺损的作用及机制研究

项目名称： 石墨烯水凝胶多孔支架修复颅面骨缺损的作用及机制研究

项目编号： No.81470714

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 邹德荣

作者单位： 上海交通大学

项目金额： 73万元

中文摘要： 颅面骨缺损严重影响患者生活质量，再生医学是解决这一临床难题的最有效途径。已知石墨烯具有良好的机械性能和骨生物活性，提示其用于骨再生的巨大潜力。我们已发表的文章首次证明石墨烯水凝胶的生物相容性和骨诱导性，然而石墨烯水凝胶用于颅面骨缺损的修复重建及其成骨机制国内外未见报道。我们预初实验发现运用石墨烯水凝胶构建的生物骨材料具有修复骨缺损能力，推测其通过MAPK/ERK通路调控其成骨活性。为验证这一假说，本课题通过：1）制备石墨烯水凝胶多孔支架，检测其理化性能及纳米形貌等特性；2）研究石墨烯水凝胶多孔支架的骨生物活性及其成骨信号通路；3）评价石墨烯水凝胶多孔支架材料修复动物颅面骨缺损的能力；4）阐明石墨烯水凝胶多孔支架材料的降解和成骨匹配度。从材料特性-分子机制-材料降解-组织修复四个层面深入探索，揭示石墨烯水凝胶多孔支架材料成骨作用、分子机制及降解能力。

中文关键词： 石墨烯；多孔支架；成骨分化；骨再生；信号通路

英文摘要： Craniofaclal bone defects seriously affect the quality of life. It is uegent to find a suitable artificial bone substitue with good mechanical properties and biological activities to repair the defect. We known that graphene has excellent mechanical properties and osteogenic activity, indicating its great potential for bone regeneration. We have published article for the first time that graphene hydrogel has osteoindcutivity and biodegradability, but the regeneration function and specific osteogenic molecular mechanisms of bone defect repair by graphene are not clear. This topic will be researched deeply to understand how graphene enhaces bone regeneration by four studies: 1) To establish graphene porous scaffold and evaluate its physicochemical properties and nano morphology; 2) The study of osteogenic ability and signal pathway of graphene; 3) The study of graphene porous scaffold as an artificial bone substitute to repair bone defect of rat skull; and to repair block bone defect of dog mandibular; 4) To elucidate clarify degradation of the graphene hydrogel material and how match to the osteogenesis. This project based on four aspects will provide theory that indicating the graphene hydrogel material functions of the reconstruction of bone defect, the degredation of material and the mechanism of osteogenic; and offer a new generation safe and effective artificial bone substitute for bone regeneration.

英文关键词： graphene;porous scaffold;osteogenic differentiation;bone regeneration;signal pathway