大黄鱼抗虫肽Pc-pis杀灭刺激隐核虫过程中细胞骨架损伤机理的研究

项目名称： 大黄鱼抗虫肽Pc-pis杀灭刺激隐核虫过程中细胞骨架损伤机理的研究

项目编号： No.41476118

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 天文学、地球科学

项目作者： 毛勇

作者单位： 厦门大学

项目金额： 87万元

中文摘要： 刺激隐核虫病（cryptocaryoniosis）是严重影响我国海水鱼类养殖的二类动物疫病，本项目组从我国海水鱼类抗菌肽基因资源库中首次发现了1种能够杀灭刺激隐核虫的抗虫肽Pc-pis。针对Pc-pis杀虫过程中膜穿孔和纤毛运动异常所带来的细胞骨架损伤机理等科学问题，本申请拟（1）利用免疫荧光和转录组分析技术从细胞、亚细胞乃至分子水平上解析细胞骨架的动态结构及分子特征，为寻找抗虫药物作用靶标或特异性抗原奠定重要基础；（2）研究Pc-pis与破坏细胞骨架的特异性药物协同杀虫的效果，为解决刺激隐核虫病等重大疾病难题寻找1种高效的抗虫肽杀虫方式；（3）研究Pc-pis杀虫过程中细胞骨架动态结构的改变和膜内钙离子等生理指标的变化，进而利用组学技术筛选并鉴定差异表达的细胞骨架蛋白和基因，阐明Pc-pis损伤刺激隐核虫细胞骨架的的形态、生理及分子机理，为抗虫肽药物后续研发提供重要的理论支撑。

中文关键词： 抗虫肽；细胞骨架；刺激隐核虫；协同作用；损伤机理

英文摘要： Cryptocaryonosis, as a Class II animal epidemic, has brought signi?cant economic losses to the marine fish culture industry in China. Fortunately, an antiparasitic peptide (Pc-pis) against Cryptocaryon irritans was first identified recently in our work. With first attention paid to scientific issues about cytoskeletal injury mechanism in our project, which arised from our previous observations on membrane perforation and abnormality of ciliary movement of C. irritans when exposed to Pc-pis, (1) the dynamic structure and molecular characteristics of cytoskeleton will be elucidated under cellular, subcellular and molecular level by using immunofluorescence technique and transcriptomic approach, which will help to provide a basis for the discovery of drug targets and specific antigen in C. irritans; (2) synergistic effects of Pc-pis and cytoskeleton-targeting drugs will be studied for the purpose of an effectively parasiticidal measure to solve the highly devastating cryptocaryoniosis by using antiparasitic polypeptide; (3) differentially expressed cytoskeletal proteins and genes will be further screened and identified with omics technology and subsequent computing.The results will help to elucidate the cytoskeletal injury mechanism of C. irritans at structural, physiological and molecular level and will offer theoretical support to the subsequent drug research and development of antiparasitic peptide.

英文关键词： antiparasitic peptide;cytoskeleton;Cryptocaryon irritans;synergistic effect;injury mechanism