免标记的SERS微阵列芯片对病原菌的高通量快速检测

项目名称： 免标记的SERS微阵列芯片对病原菌的高通量快速检测

项目编号： No.21505053

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 周海波

作者单位： 暨南大学

项目金额： 21万元

中文摘要： 病原菌引起的食源性疾病是国内外食品安全领域的突出问题，开发低成本、高灵敏度和高通量的病原菌快速检测方法已刻不容缓。表面增强拉曼散射（SERS）在病原菌免标记快速灵敏检测方面具有巨大的优势，但存在信号重现性差及无法实现高通量检测等问题。本项目拟探索在病原菌表面原位合成纳米粒子的方法，据此构建免标记的SERS微阵列芯片检测系统，实现对多种病原菌的高通量检测。（1）发展纳米粒子在病原菌表面原位合成的方法，通过病原菌自身SERS信号的输出，探索纳米粒子的最佳合成条件及信号获取途径；（2）将原位合成方法应用于微阵列芯片上，探索SERS微阵列芯片对多种病原菌免标记的拉曼特征识别，实现多种病原菌的拉曼信号同步稳定读出；（3）结合化学计量学方法，建立SERS微阵列芯片数据分析与病原菌快速区分算法，搭建用于病原菌高通量快速检测的分析平台。本项目的预期成果将为病原菌的快速及高通量分析提供科学依据与技术途径。

中文关键词： 免标记；表面增强拉曼散射；微阵列芯片；病原菌；高通量检测

英文摘要： Foodborne diseases caused by pathogens represent major issues in food safety. It is urgently demanded to develop a low-cost, high sensitivity and high-throughput method for rapid detection of pathogens. Surface-enhanced Raman scattering (SERS) shows excellent merits in rapid, label-free and sensitive detection of pathogens. However, it is hard to get reproducible SERS signals and to achieve high-throughput detection. This project aims at exploring the in-situ synthesis of SERS nanoparticles on the surface of pathogens, then building SERS based label-free microarray readout system for high-throughput and rapid detection of multiple pathogens. (1) Develop and optimize the in-situ synthesis method of nanoparticles on the pathogens surface, and explore the best approach for signals acquisition; (2) stimultaneous detect a variety of pathogens based on the stability and specialty of Raman signal readout on the microarray; (3) By combining with chemometrics, establish algorithm for SERS microarray data analysis and pathogens quickly distinguish, and bulid a high-throughput platform for the rapid detection of pathogens. This project will provide a scientific basis and technical approach for fast and high-throughput analysis of pathogens.

英文关键词： label-free;SERS;microarray;pathogens;high-throughput detection