应力负荷介导下Ihh/PTHrP信号轴对髁突前软骨干细胞分化的影响及作用机制

项目名称： 应力负荷介导下Ihh/PTHrP信号轴对髁突前软骨干细胞分化的影响及作用机制

项目编号： No.81200802

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学二处

项目作者： 冯剑颖

作者单位： 浙江中医药大学

项目金额： 23万元

中文摘要： 髁突软骨的适应性改建是影响颞下颌关节紊乱病患者病程转归的关键因素。髁突软骨内储存的前软骨干细胞长期保持向软骨细胞定向分化的潜能，在髁突形成、生长发育和软骨损伤后修复中具有重要作用。本研究采用激光共聚焦显微镜、显微CT、免疫磁珠分选技术、流式细胞仪、蛋白质和核酸分析等研究技术，分析生长发育期不同咀嚼应力负荷对兔髁突前软骨干细胞的分布、分化和增殖的时空表达的影响，寻找咀嚼应力负荷和Ihh/PTHrP信号轴的内在联系，探讨间歇静压力介导下Ihh/PTHrP信号轴通路对髁突前软骨干细胞向软骨细胞定向分化的作用机制。本研究有助于探寻如何在异常应力负荷下适时启动髁突前软骨干细胞分化增殖，引导髁突软骨实现自发性的适应性改建，从而为颞下颌关节紊乱病提供新的治疗思路和方法。

中文关键词： 颞下颌关节；前软骨干细胞；应力；；

英文摘要： The remodeling of condyle cartilage was one of the key points which influenced the prognosis of temporomandiublar joint disorder. The precartilaginous stem cells (PSCs) were found in condyle for long-term, remaining the differentiation porential even in adult. PSCs played an important role in the development of condyle and cartilage remodeling. The objective of this research was to detect the effect of masticatory load on the distribution, differentiation and proliferation of PSCs. We attempted to found the mechanism and relationship of the intermittent hydrostatic pressure and Ihh/PTHrP signal pathway on the differentiation of PSCs, by the methods of the laser scanning confocal microscope, micro-CT, immunomagnetic separation, flow cytometry, Western Blot and RT - PCR.The research would be benefit to find out the pathway of the differentiation of PSCs when the patients were suffered with the abnormal stress load such as temporomandibualar joint disorder. How to induce the adaptive remodeling would be critical to clinical treatment of TMD.

英文关键词： Temporomandibular joint；PSCs；Pressure；；