小麦种子VP1/ABA分子调控网络与穗发芽抗性机理研究

项目名称： 小麦种子VP1/ABA分子调控网络与穗发芽抗性机理研究

项目编号： No.31271717

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 黄涛

作者单位： 华中农业大学

项目金额： 83万元

中文摘要： 脱落酸(ABA)在种子发育过程中形成复杂的调控网络，VP1处于其中的核心位置，参与调控种子发育、休眠和穗发芽抗性。小麦Vp1基因转录本错拼而丧失调控功能，导致小麦容易发生穗发芽。我们首次将玉米Vp1基因及其调控序列导入小麦，获得了稳定遗传的高抗穗发芽转基因小麦品系，证实外源Vp1基因可以补偿小麦的功能。但外源Vp1基因参与ABA调控网络的机制并不清楚。本研究以小麦基因芯片、双向电泳和核磁共振技术分析发育过程中以及在ABA处理下转基因品系与未转化对照间小麦胚在转录组、蛋白质组和代谢组上的差异，鉴定受Vp1调控的基因和代谢途径，鉴别VP1/ABA调控网络的主要节点；结合qRT-PCR和拟南芥突变体验证关键节点上基因的功能。本项目的完成，将从分子水平揭示VP1/ABA调控网络及其与小麦种子发育和穗发芽抗性的内在联系，为精确调控小麦种子发育和穗发芽抗性奠定理论基础，为培育小麦抗性品种提供候选基因。

中文关键词： 小麦种子；穗发芽；VP1；脱落酸；调控网络

英文摘要： VP1 plays a key role in ABA regulatory network, and participates in seed development, dormancy and preharvest sprouting tolerance. Wheat Vp1 gene loses its regulation function because of misspicing of pre-mRNA which is regarded as the main factor in wheat preharvest sprouting susceptibility. We have introduced a maize Vp1 gene with its own promoter into wheat and obtained stable heritable transgenic lines with strong preharvest sprouting tolerance. The results demonstrated for the first time that the exogenous Vp1 gene can compensate wheat Vp1 function. However, the regulation pattern of exogenous Vp1 gene in ABA regulatory network in wheat is not clear. The proposed study will investigate the differences of transcriptome, proteome and metabolomics between Vp1 transgenic lines and non-transgenic control during seed development and under ABA treatment of excised embryos by micorarray, two-dimensional electrophoresis and nuclear magnetic resonance analysis. Combination of these data will identify the main genes, metabolite patnways and key nodes in VP1/ABA signaling network.The genes at the main nodes will be verified by qRT-PCR and Arabidopsis thaliana mutants. When the study is completed, the regulatory network and pattern of VP1/ABA as well as the relationship of the regulatory network with seed development and

英文关键词： Wheat seed；Preharvest sprouting；VP1；ABA；Regulatory network