家蚕丝素小口径人工血管优化设计及其原位诱导血管组织重建

项目名称： 家蚕丝素小口径人工血管优化设计及其原位诱导血管组织重建

项目编号： No.51473108

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 一般工业技术

项目作者： 王建南

作者单位： 苏州大学

项目金额： 83万元

中文摘要： 针对目前小口径人工血管存在血栓和再狭窄的关键问题，以及力学性能和降解的适配性问题，根据血管微结构特征和功能，以γ-射线处理的丝素管状织物为芯层，渗透并包覆再生丝素蛋白多孔材料，同时利用丝素蛋白优越的极性基团引入功能肽或功能因子，优化构建一种三层仿生小口径管状支架。研究管状支架的细胞毒性、细胞相容性、血液相容性、组织相容性、生物降解性和近远期通畅率；优化平滑肌细胞和内皮细胞共培养的竞争性、体内快速内皮化能力和抗凝血性能；探究原位诱导血管重建的能力，以及降解速率调控、力学性能与血管重建的适配机制。构建的管状支架内部为三维连孔结构，利于平滑肌细胞和成纤维细胞长入，内表面为分布有少量纳微孔的粗糙界面，利于内皮细胞粘附；并赋予了支架原位快速诱导内皮化、抑制增生和抗凝血功能的修饰，以及优异的力学性能与降解可控性；最终恢复血管自身功能，达到自然血管的完整性和功能性。期望填补小口径人工血管的临床空白。

中文关键词： 生物医用材料(人工血管)；生物相容性；血液相容性；生物降解性；血管重建

英文摘要： In order to solve the key problems of thrombosis and restenosis, and the suitability problems of mechanical properties and degradation of artificial blood vessels, a novel biomimetic silk fibroin tubular scaffold (SFTS) is designed consisting of three distinct layers: a regenerated SF intima, a silk braided media treated by γ-ray and a regenerated SF adventitia. Meanwhile, some functional peptides or functional factors are introduced into SF molecules. Studying the cytotoxicity, cytocompatibility, blood compatibility, tissue compatibility, biodegradation and long-term patency rate of the SFTS. Optimizing the competitive ability of smooth muscle cell and endothelial cell co-culture, the quickly endothelial ability and the anticoagulation capability. Exploring the ability of in situ induced vascular remodeling, and the adaptation mechanism between the mechanical properties, degradation rate and vascular remodeling. Interior of the SFTS is 3-D pore structure for ingrowth of the smooth muscle cells and fibroblasts, inner surface is coarse interface with nano-micron pores for adhesion of endothelial cells. The SFTS is modified to endow the functions for quickly endothelialization, inhibition of intimal hyperplasia and anticoagulation, and endow the excellent mechanical properties and controllable degradation. The ultimate objective is to renew the functions of blood vessels themselves, and to achieve the integrity of the natural blood vessels, expecting to fill the gap of the small diameter artificial blood vessel in clinical.

英文关键词： Biomedical materials(Artificial blood vessels);Biocompatibility;Blood compatibility;Biodegradability;Vascular remodeling