植物病毒的微流控芯片ELISA智能便携平台测定方法研究

项目名称： 植物病毒的微流控芯片ELISA智能便携平台测定方法研究

项目编号： No.21505061

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 吴晶

作者单位： 兰州大学

项目金额： 21万元

中文摘要： 植物病毒严重威胁着农作物的产量和品质，随着我国农业进出口贸易的增加，外来植物病毒传入我国的风险也越大，因此迫切需要检测效率高且能够在现场使用的便携式植物病毒检测装置进行检测工作。本项目拟结合微流控技术和ELISA来实现这一目标。通过设计加工螺旋式微通道芯片，利用光化学改性的方法对其内表面改性接枝氨基化合物，优化光化学改性条件和抗原-抗体反应条件，利用目前普及度很高的平板电脑作为检测平台，用其自身的摄像头作为比色检测的工具，拍摄显色图片，找出图片的RGB颜色值与待测物浓度的关系，实现ELISA检测。编写数据处理软件，使最终结果能够实时显示在平板电脑上，并可通过蓝牙或WIFI等无线通信方式将结果传输至其它手机、电脑或Internet，以实现数据汇总。研究结果将以植物病毒检测的微流控芯片一体化检测平台的方式呈现，可为微流控检测技术与现代民用电子产品的结合奠定技术基础。

中文关键词： 微流控技术；植物病毒；酶联免疫吸附；平板电脑；比色检测

英文摘要： The yield and quality of crop are seriously threatened by plant virus, with the increasing of import & export trade of China’s agriculture, the risks brought by plant virus from other country become greater and greater. So it is an urgent requirement to develop portable equipment with high detection efficiency for detecting plant virus. The project intends to combining microfluidic technology with ELISA to achieve the targets, which including: Desiging and machining microchips with the spiral channel, which needed to be modified by compounds with amino-group through ultraviolet initiated polymerization; Optimizing modification condition of ultraviolet initiated polymerization and reaction condition of antigen-antibody; Taking advantages of popular tablet PC as detection platform, and its own camera could be used for colorimetric detection, finding the relationship between RGB value of pictures and the concentration of determinand to achieve ELISA assay; Programming the software of date processing, so that the final results could be displayed on a tablet PC at real-time, and it could be sent to other smart phones, computers or Internet through Bluetooth and WIFI, which might provide basis for data aggregation. The results will be taken on in form of microfluidic chips of integration platform for plant virus detecting, which can lay the foundation for combination between microfluidic technology and modern civil electronic products.

英文关键词： Microfluidic technology;plant virus;ELISA ;Tablet PC;Colorimetric detection