植物MIR156a调控血管F11R/JAM-A表达在动脉粥样硬化发病中的功能研究

项目名称： 植物MIR156a调控血管F11R/JAM-A表达在动脉粥样硬化发病中的功能研究

项目编号： No.31301060

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 侯冬霞

作者单位： 南京大学

项目金额： 24万元

中文摘要： 动脉粥样硬化是一种危害人类健康的常见病，是缺血性心脏病等高致死率疾病的发病基础。F11R/JAM-A在动脉粥样硬化发病过程中起了重要的调节作用。MicroRNAs 是一类非编码小分子RNA，它们以碱基互补的形式与靶基因mRNA结合，以抑制靶基因蛋白翻译或降解靶基因mRNA 的方式降低靶基因蛋白的表达，参与调控多种疾病。我们的前期实验证明，外源性食物miRNAs稳定存在于哺乳动物血清与组织中，其中植物MIR156a和MIR168a含量最多；进一步研究表明食物中的植物MIR168a能被摄食者吸收并运送到其血液和肝组织里，跨界调控哺乳动物靶基因LDLRAP1的表达，进而影响生理和病理功能。本项目主要研究植物MIR156a与靶基因F11R/JAM-A的调控关系，并进一步探讨MIR156a对动脉粥样硬化发生发展的调节作用，为植物miRNA的跨界调控机制及动脉粥样硬化的预防与治疗提供新的理论依据。

中文关键词： 植物MIR156a；连接黏附分子；动脉粥样硬化；；

英文摘要： It is well known that athrosclerosis is the underlying mechnism of ischemic heart disease and stroke, which are the leading cause of death in the world today. The previous studies have determined that the F11 receptor (F11R; aka JAM-A) plays an important role in the initiation formation and subsequent growth of atherosclerotic plaques. MicroRNAs are small noncoding RNAs that are involved in various diseases. They regulate protein expression by base-pairing to the complementary sites on the target mRNAs,thus blocking the translation or triggering the degradation of the target mRNA. In the previous study, we were surprised to detect exogenous plant miRNAs in the serum and plasma of human and animals, especially plant MIR156a and MIR168a. Further in vitro and in vivo analysis demonstrated that food-derived exogenous plant MIR168a can pass through the mouse gastrointestinal (GI) track and enter the circulation and various organs especially the liver where it cross-kingdomly regulates mouse low density lipoprotein receptor adapter protein 1 expression and physiological condition. The purpose of this study is to determine whether MIR156a regulates the expression of mammalian F11R/JAM-A and prevents atherosclerotic plaques from forming and growth. These findings raise both speculation about the potential impact that pl

英文关键词： plant MIR156a；JAM-A；atherosclerosis；；