金属型纳米颗粒与重金属的交互作用对丛枝菌根的毒性效应及其吸收和转运

项目名称： 金属型纳米颗粒与重金属的交互作用对丛枝菌根的毒性效应及其吸收和转运

项目编号： No.41471395

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 地质学

项目作者： 王发园

作者单位： 青岛科技大学

项目金额： 90万元

中文摘要： 丛枝菌根是自然界中普遍存在的植物-真菌共生体，对于改善宿主植物的矿质营养和抗逆性、参与碳、氮、磷等元素循环、维持土壤健康和植物群落的稳定性及生产力有重要作用。金属型纳米颗粒（MNPs）和重金属污染物的生物效应均受到广泛关注，但是二者交互作用与丛枝菌根之间的关系尚缺乏研究。本课题围绕MNPs、重金属和丛枝菌根，利用高通量蛋白组学技术、PCR-DGGE等深入研究（1）MNPs和重金属交互作用对丛枝菌根的生物效应及毒性机理；（2）MNPs和重金属交互作用条件下菌根植物的吸收、转运及解毒机制；（3）MNPs和重金属交互作用条件下其在植物和根外菌丝内的形态和分布特征。研究目标是揭示MNPs与重金属交互作用的菌根毒性及丛枝菌根的解毒机制，阐明MNPs、重金属在丛枝菌根中的吸收、转运和积累规律。研究结果将加深对土壤生态系统中MNPs生物效应和安全性的认识和理解，为生物修复和改善农产品安全提供理论依据。

中文关键词： 纳米颗粒物；重金属；土壤污染；丛枝菌根；转运

英文摘要： As the most widely distributed mutualistic symbiosis between plants and fungi in nature, arbuscular mycorrhizae play important roles in improving mineral nutrition and resistance of host plants, contributing to nutrient cycling of carbon, nitrogen, phosphorus and other elements, and maintaining soil health and plant community stability and productivity. The biological effects of metal-based nanoparticles (MNPs) and heavy metal pollutants have attracted wide attention, but little is known on their interaction and the relationship with arbuscular mycorrhizae. Using high-throughput proteomics and PCR-DGGE techniques, the present project aims to focus on MNPs, heavy metals and arbuscular mycorrhizae, and will deeply study: (1) interactive biological effects of MNPs and heavy metals to arbuscular mycorrhizae and toxicity mechanisms; (2) interaction of MNPs and heavy metals on their uptake and transport in mycorrhizal plants and the detoxification mechanisms; and (3) interaction of MNPs and heavy metals on their forms and distribution characters in plants and extraradical mycelia. Our aims are to reveal the interactive toxicity of MNPs and heavy metals to arbuscular mycorrhizae and their detoxification mechanisms, and to elucidate the uptake, transport and accumulation of MNPs and heavy metals in arbuscular mycorrhizae. The results will help deeply recognize and understand the biological effects and safety of MNPs in soil ecosystem, and provide theoretical basis for bioremediation and improving the security of agricultural products.

英文关键词： Nanoparticle;Heavy metal;Soil pollution;Arbuscular mycorrhizae;Transport