特络细胞与肾小管生发前体细胞胞间对话在急性肾损伤修复中的作用和机制

项目名称： 特络细胞与肾小管生发前体细胞胞间对话在急性肾损伤修复中的作用和机制

项目编号： No.81500570

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 戚贵生

作者单位： 复旦大学

项目金额： 18万元

中文摘要： 急性肾损伤修复的细胞来源及机制尚未明确。特络细胞（Telocyte, TC）是新近发现的一种形态、表型独特的细胞，广泛分布于心、骨骼肌、皮肤等器官中。我们于2012年率先发现并报道了肾脏特络细胞，描述了其形态特征和分泌功能，推测其可能为干细胞孵育细胞。随后我们发现过继输注肾脏特络细胞，可减轻肾脏缺血再灌注损伤，保护肾功能，且其作用途径依赖于体内条件下生长因子的释放，进一步验证了我们的推测。与此同时，其他研究也表明，心脏中的特络细胞存在于心肌干细胞龛中，发挥孵育功能，修复心肌损伤。研究表明，肾脏CD24+CD133+CD106-肾小管生发前体细胞具备自我更新和多向分化能力，是肾脏内源性干细胞。本项目拟在前期研究基础上，通过体内外试验，观察特络细胞能否促进肾小管生发前体细胞的增殖及向肾小管上皮细胞定向分化；并进一步通过蛋白谱研究，探讨两者之间对话的关键分子，为急性肾损伤修复提供新的思路和策略。

中文关键词： 特络细胞；肾小管生发前体细胞；急性肾损伤；肾移植；损伤修复

英文摘要： Cell resources and mechanisms involved in the process of acute renal injury reparation are still unclear. Telocyte, a special cell with unique morphology and phenotype, has been unveiled in many organs, such as heart, skeletal muscle and skin. In 2012, we reported the existence in human renal cortex for the first time in worldwide. We successfully defined the morphologic characteristics and secretion function which indicated that telocyte could be a potential stem cell supporting cell. Furthermore, we found that infusion of renal telocytes into rats suffering acute renal injury were able to reduce the ischemia/reperfusion injury and preserve the renal function, which were dependent on the growth factor releasing effect in vivo. This phenomenon verified our speculation to a solider degree. Meanwhile, other researches proved that telocytes, as supporting cells, were located in myocardium stem cell niches, protecting myocardium cells from injury. On the other hand, previous studies have confirmed that CD24+CD133+CD106- tubular progenitor cells with the capability of self-renewal and pluripotent differentiation were potential renal stem cells. Based on our former work and subsistent foundings, we plan to verify whether telocytes can promote the proliferation and tubular lineage committed differentiation of CD24+CD133+CD106- renal progenitor cells in vivo or ex vivo, and explore the key molecular participating in the cross-talk between telocyte and renal progenitor cell utilizing proteinogram. The program is aimed to provide new approaches andstrategies for acute renal injury.

英文关键词： telocyte;tubular progenitor cell;acute renal injury;renal transplantation;injury impairment