电磁场对海马神经元TRP离子通道作用研究

项目名称： 电磁场对海马神经元TRP离子通道作用研究

项目编号： No.31270890

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 钟敏

作者单位： 中国人民解放军第三军医大学

项目金额： 80万元

中文摘要： 电磁辐射的健康效应备受关注，认识细胞对电磁场作用的感受及其信号转导是阐明电磁辐射生物效应发生机制的重要基础。本项目定位在从离子通道途径认识细胞对电磁场作用的响应。研究以海马神经元为对象，钙离子通道为切入点，应用膜片钳、荧光探针细胞内钙离子成像等技术，在研究电磁场对海马神经元电压门控钙离子通道电生理活动影响及其作用特征的基础上，进一步研究了瞬时受体电位通道（transient receptor potential channels,TRP channels）-一种具有多种感受功能的新型钙通道，对电磁场作用的响应及其介导的细胞钙信号转导。通过本项目研究，希望对揭示以下科学问题提供重要依据：①离子通道在细胞感受并转导电磁场信号这一分子事件中的作用；②TRP通道对电磁场作用的感受性及其特征；③TRP通道在介导电磁场作用下神经元钙信号转导中的作用。

中文关键词： 电磁场；神经元；钙离子通道；TRPC；VGCC

英文摘要： Primary effect process of electromagnetic field on cells has been given broad attention for the health effect of electromagnetic radiation.Understanding the response and signal transduction of cells to electromagnetic field is the important basis to clarify the biological effect mechanism of electromagnetic radiation.This program focuses on understanding of how cells responses to electromagnetic field through ion channels. The research, which takes hippocampus neurous as object of study, Ca2+ ion channels as point of penetration, takes advantage of techniques such as patch clamp recording technique and fluorescence probe inner cell calcium imaging, based on the study of Electrophysiological properties of voltage-dependent calcium channels in hippocampus neurous, we take further researches about transient receptor potential channels(TRP channels) , which refers to a new kind of Ca2+ ion channel with multiple sensing functions, its susceptibility to electromagnetic field and the function of mediated cell calciun signal transduction. We hope the following scientific questions can be answered clearly by this researches: ①The role played by ion channels in cell sensing and transmitting electromagnetic signal; ②TRP's susceptibility and characteristics caused by electromagnetic filed; ③The role played by TRP channel

英文关键词： EMF；hippocampus neurous；Ca2+ channel；TRPC；VGCC