群体感应对土传病害高效生防菌--多粘类芽孢杆菌生物膜形成的影响及其与防效之间的关系

项目名称： 群体感应对土传病害高效生防菌--多粘类芽孢杆菌生物膜形成的影响及其与防效之间的关系

项目编号： No.31501693

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 罗远婵

作者单位： 华东理工大学

项目金额： 20万元

中文摘要： 为解决化学农药带来的食品安全及环境污染问题，微生物农药因其高效、无污染等优点，近年得到快速发展。生防菌的定殖能力是决定微生物农药能否正常发挥药效的首要条件。在植物根围形成生物膜是防治土传病害生防菌具有良好定殖能力的重要指征，生物膜的形成有利于防效的提高。细菌的自调节系统--群体感应（QS）可以影响生物膜的形成。迄今，QS对多粘类芽孢杆菌生物膜形成的影响及其与防效之间关系未见报道。.本项目拟以国内外首创并已实现产业化的防治青枯病等土传病害的多粘类芽孢杆菌系列微生物农药的生防菌—多粘类芽孢杆菌HY96-2为对象，通过多粘类芽孢杆菌-番茄-青枯病体系，首次开展QS对多粘类芽孢杆菌生物膜形成的影响研究，进而明晰QS与HY96-2防效之间的相关性。本研究不仅可进一步阐明HY96-2防治土传病害的机制，而且可为多粘类芽孢杆菌农药应用技术的优化提供科学依据，对推动其在土传病害防治中的大规模应用具有重要意

中文关键词： 群体感应；多粘类芽孢杆菌；生物膜；土传病害；防效

英文摘要： To solve the problems of food safety and environment pollution bring by chemical pesticide, micro-pesticide has been developed rapidly in recent years for the advantages of high control efficacy, environment friendly and the safety of animal and human. The control efficacy of micro-pesticides mostly depend on the colonization capacity of the biocontrol agent. Forming biofilm on plant root is important indicate of good colonization capacity of the biocontrol agent for controlling soil-borne disease. Biofilm formation facilitate the promotion of control efficacy of biocontrol agentor controlling soil-borne disease. Regulating biofilm formation is one important function of quorum sensing, a self modulation system of bacteria. There are not any research about the effect on biofilm formation and control efficacy of Paenibacillus polymyxa by quorum sensing have been reported..Here we proposed a project to reveal the effect on biofilm formation and control efficacy of P. polymyxa by quorum sensing with P. polymyxa HY96-2 strain, the biocontrol agent of commercialized P. polymyxa pesticide. The relationship between biofilm formation, control efficacy of P. polymyxa and quorum sensing will be researched firstly by the P. polymyxa（mutant strain）-tomato-bacteria wilt system. The results of this program will further clarify the biocontrol mechanism of P. polymyxa HY96-2, and provide scientific evidence for the optimization of application technic of P. polymyxa pesticide, which is important for the promotion of controlling soil-borne disease with P. polymyxa pesticide in large scale.

英文关键词： Quorum sensing;Paenibacillus polymyxa;Biofilm;Soil-borne disease;Efficacy