兔骨骼肌损伤修复的超声影像定量诊断

项目名称： 兔骨骼肌损伤修复的超声影像定量诊断

项目编号： No.81501492

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 赵佳琦

作者单位： 中国人民解放军第二军医大学

项目金额： 18万元

中文摘要： 创伤打击致骨骼肌钝挫伤较常见，超声检查是其定性诊断的首选影像学方法。离体研究发现，损伤后骨骼肌纤维结构破坏可致超声图像发生变化，但不同技术级别超声医师单独进行定性诊断时具有主观局限性，目前临床对于随访骨骼肌损伤及修复期的超声影像演变尚未形成可靠定量诊断标准。故本项目拟建立重物打击致兔骨骼肌损伤模型，以灰阶超声、彩色多普勒血流成像、超声造影等多模式超声影像资料作为研究对象，①运用计算机纹理定量分析研究伤肢骨骼肌束损伤程度，探讨损伤后纹理特征与病理学改变的相关规律；②利用贝叶斯跟踪框架法跟踪超声造影灌注视频图像中血管束的形成，从血管束几何形状和空间排列分布两方面对造影图像进行动态定量分析，探讨伤肢肌群血流灌注特征与微循环演变。旨在从肌束纹理结构和血管束架构变化角度入手，定量阐明兔骨骼肌损伤修复不同时期病变发生发展及特征性超声影像演变特征，为临床寻找定量、客观的超声诊断评价指标提供理论依据。

中文关键词： 骨骼肌；损伤；超声检查；纹理分析；定量超声

英文摘要： Skeletal muscle can be severely injured by contusion, which induced muscle fibers degeneration, necrosis, regeneration, fibrosis or nourishing blood vessels variation, thrombosis, occlusion,ect. Although ultrasonography is regarded as an invasive and objective diagnostic method with highest sensitivity for muscle injury, there is still lack of an ideal quantitative method to assess the imaging findings. Multimode ultrasound can be used to demonstrate injured muscle texture and framework of its nourishing vessels, both of them contribute to special features based on ultrasonograms. In this study, we aim to establish experimental rabbit models of skeletal muscle injured by acute contusion, and to investigate the corresponding ultrasonographic feature evolutions and pathological changes deeply. Contrast Enhanced Ultrasound(CEUS) plays an important role in observing microcirculation perfusion of the injured muscle, so specific imaging characteristics can be dynamically observed and quantitatively analyzed with computer-assisted image analysis technique. The corresponding muscle texture feature and vascular bundle geometry extracted from the region of interest (ROI) from either case will be compared statistically, which will be helpful to guide the explorative quantitative ultrasound diagnosis and evaluation, in order to provide complementary information which could potentially help in skeletal muscles injury and regeneration characterization．

英文关键词： skeletal muscle;injury;ultrasonography;texture analysis;quantitative ultrasound