SPR超光谱荧光生物芯片检测方法及其应用研究

项目名称： SPR超光谱荧光生物芯片检测方法及其应用研究

项目编号： No.61275188

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 无线电电子学、电信技术

项目作者： 何永红

作者单位： 清华大学

项目金额： 88万元

中文摘要： 近年来不断发展的生物芯片技术，通过对基因表达图谱进行数据挖掘，能够有效地诊断临床表征不明的多种疾病。随着生物芯片技术的不断革新，对生物芯片的检测也提出了越来越高的要求，以往单一的检测方法已经很难适应生物芯片的检测需求。本课题提出了一种能够同时实现荧光与表面等离子体共振（surface plasmon resonance, SPR）成像的生物芯片检测方法。在荧光检测部分，采用超光谱成像机制，通过记录生物芯片上每一点的光谱，有效去除干扰信号的影响，提高检测结果的准确性。在SPR检测部分，采用角度谱型SPR方法，并通过偏振干涉技术降低噪声水平，实现高灵敏度、高折射率分辨率的SPR成像。系统采用线扫描的成像方式，提供了对生物芯片的高通量检测。检测结果同时具有荧光和SPR信号，不但实现了对生物芯片的多参量分析，而且两种信号可以为彼此提供参照，排除假阳性、假阴性等情况，从而有效地应用于疾病早期诊断。

中文关键词： 表面等离子体共振；生物芯片；超光谱荧光成像；；

英文摘要： The advent of microarray technology has revolutionized the biomedical research in genetic engineering. By exploring the gene expression profile, the microarray technology has been efficiently applied to the early diagnosis of desease. For many microarrays, several biomolecular interactions occur simultaneously and the interplay of various factors that affect these interactions remains poorly understood. Detecting such interactions with a single technique can often be a difficult and complicated process. In this project we propose a combined technique which enables simultaneous fluorescence imaging and surface plasmon resonance (SPR) sensing. In the part of fluorescence imaging, a hyperspectral imaging mechanism is adopted. Such an imaging mechanism records the entire emission spectrum for every voxel within the imaged area, so that it allows for quantitative identification of the contributions of multiple fluorescent dyes and elimination of unwanted artifacts. In the part of SPR sensing, the angle-interrogation SPR is modulated with the polarization interferometry technique to improve sensing performance and reduce restrictions on allowable parameters of SPR-supporting metal films. This tandem technique offers two-dimensional imaging of the whole array plane by a line scanning architecture. The refractive index

英文关键词： Surface PlasmonResonance；Miccrarray；Hyperspectral Fluorescence Imaging；；