紫苏醛对食品储藏中黄曲霉的抑制作用及其机理研究

项目名称： 紫苏醛对食品储藏中黄曲霉的抑制作用及其机理研究

项目编号： No.31301585

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 田俊

作者单位： 江苏师范大学

项目金额： 24万元

中文摘要： 黄曲霉引起的食品腐败变质已为全球关注的重大食品安全问题。紫苏醛（PEE）作为绿色安全化合物其抗真菌活性显著，但作用机理尚未阐明。本项目拟通过研究PEE对菌丝生长等体外指标评价抗黄曲霉活性，同时利用气相防腐技术评价体内抗黄曲霉活性，在此基础上系统进行作用机理研究：1）明确PEE抗菌靶标，分析PEE在细胞水平上对麦角固醇合成、细胞膜损伤、细胞核物质泄漏的影响，在亚细胞水平上对线粒体ATP酶、SDH酶、MDH酶、膜电位及ROS累积的影响，在分子水平上对DNA和RNA损伤的影响；2）以线粒体损伤为切入点，通过DAPI染色、Annexin V染色、Caspase活性、Cyt C的释放等研究揭示其凋亡途径；3）基于PEE对核酸分子损伤，通过周期蛋白基因表达水平的变化以探明PEE对细胞周期的阻滞阶段。本项目通过以上研究阐明PEE抑制黄曲霉的作用及机理，将为新型天然食品防腐剂的应用与开发提供理论依据。

中文关键词： 紫苏醛；黄曲霉；抗真菌；作用机理；

英文摘要： Food spoilage caused by Aspergillus flavus is becoming a major food safety issue of global concern. As a natural green safe compound, perillaldehyde (PEE) showed significant antifungal activities, however, its mechanism of action has not yet been ascertained. Therefore, the present project will evaluate the antifungal activities of PEE through measuring various in vitro markers, such as mycelial growth, and also assess in vivo antifungal activities by exposing it to PEE vapor. On top of that, systematic study on the mechanism of antifungal action of PEE against A. glavus will be conducted. 1) To determine the exact target of PEE, at the cellular level, the effects of PEE on inhibition of ergoterol synthesis, lesion in the plasma membrane and release of cellular material will be investigated; at the subcellular level, the effects of PEE on mitochondrial ATPase, succinate dehydrogenase (SDH), malate dehydrogenase (MDH), membrane potential and ROS accumulation will be assessed; at the molecular level, the effects of PEE on the damage of DNA and RNA will be evaluated. 2) Based on the mitochondrial lesion, DAPI staining, Annexin V staining, metacaspase activity and release of cytochrome c (Cyt C) will be investigated to identify an apoptosis pathway in A. flavus. 3) Based on the DNA or RNA lesion, PEE-induced cell cy

英文关键词： perillaldehyde；Aspergillus flavus；antifungal；mechanism；