基于分子印迹有机磷水解模拟酶的仿生传感器

项目名称： 基于分子印迹有机磷水解模拟酶的仿生传感器

项目编号： No.21275097

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 张耀东

作者单位： 陕西师范大学

项目金额： 80万元

中文摘要： 本项目提出：既利用分子印迹技术稳定有机磷水解的过渡态分子，又整体仿生模拟天然有机磷水解酶（OPH）的活性中心，合成稳定性优于天然OPH的仿生OPH，并作为分子识别元件构建有机磷仿生传感器，以提高有机磷传感器的稳定性和实用性。首先根据天然OPH各功能团间协同催化水解机制，设计合成含识别、结合和催化等功能团的双核金属离子配合物，并作为功能单体。同时，设计合成含起协同催化作用功能团的辅助单体。然后，以过渡态类似物为印迹模板，采用基于化学计量非共价作用的分子印迹法，整体仿生印迹天然OPH起识别、结合和催化作用的各功能团。研究仿生酶对有机磷底物的催化水解活性，并借助柔性分子对接方法揭示OPH与其配体的相互作用，探索功能团对仿生酶活性影响的规律。最后，研究将仿生酶共价固定于一次性印刷电极、聚苯乙烯微孔板上的方法，分别研究并完善有机磷仿生传感的电化学及光化学检测模式，建立有机磷仿生检测的新方法。

中文关键词： 仿生酶；仿生传感器；分子印迹；有机磷水解酶；有机磷

英文摘要： We propose a molecularly imprinted biomimetic enzyme with organophosphorus hydrolase (OPH)-like activity in order to develop the biomimetic sensors for neurotoxic organophosphate with enhanced stability and practicality. In this proposal, not only will the shape of the transition state of organophosphate be mimicked by imprinting but also at the same time appropriate catalytic groups and binding sites of natural OPH will be integrated into the active site in a predetermined orientation of the biomimetic OPH. The stable biomimetic OPH will be designed in accordance with the activity center of fragile natural OPH, prepared by the molecular imprinting technique and employed as a molecular recognition element. A binuclear metal ions complex will be designed to mimic the recognition, binding and catalytic functional groups of the activity center of natural OPH. Then, the designed complex will be synthesized and used as functional monomers. Meanwhile, the auxiliary monomer will be also designed to mimic the cooperative catalytic effect and synthesized. After that, the transition-state analogues of the organophosphate hydrolysis will be selected or designed as templates to incorporate the main catalytic elements. Thus, the biomimetic enzyme with organophosphorus hydrolase-like activity will be imprinted in term of sto

英文关键词： Biomimetic enzyme；Biomimetic sensor；Molecular imprinting；Organophosphorus hydrolase；Organophosphate