地理格局及生态驱动揭示肉苁蓉品质生态型机理

项目名称： 地理格局及生态驱动揭示肉苁蓉品质生态型机理

项目编号： No.81473315

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 黄林芳

作者单位： 中国医学科学院药用植物研究所

项目金额： 73万元

中文摘要： 道地药材品质特征及形成机理是重要科学难题。申请人前期研究表明肉苁蓉 (Cistanche deserticola)品质分化为新疆和内蒙古两大生态型，且品质差异与地理分布格局相关。据此我们提出假说肉苁蓉品质变异是在特定气候地理区域生态驱动与适应的结果。本研究整合空间分子生态学、代谢组学及生物信息学方法,开展其品质变异的生态驱动和生态策略研究。应用中药材生态适宜性TCMGIS集成技术、演化建模等方法探讨生态特征；UPLC-Q-TOF/MS等分析方法揭示品质特征；从梭梭-肉苁蓉互惠寄生的生态适应组合表征诠释对肉苁蓉品质的影响；在已获得的转录组数据基础上开发SSR、SNP标记，分析居群空间遗传变异结构，发掘影响品质变异的分子地理标识，揭示关键驱动生态因子。本研究对肉苁蓉特色药材生产中筛选优良种质资源，适生地选择及GAP规范化种植提供理论基础，为道地药材生态型变异及生态适应的生物学本质提供新视角

中文关键词： 品质评价；分子鉴定；药用植物

英文摘要： The quality characteristics of Dao-di herbs and its formation mechanism is an importantl scientific issue. Our pilot studies demonstrated that Cistanche deserticola can be divide into Xinjiang and Inner Mongolia tow chemo-ecotypes by chemical compound profiles and psbA-trnH, and the quality variation is related to geographical distribution pattern. Accordingly，we hypothesize that the ecological driving and eco-adaptability under the specific climatic geographic zone lead to quality variation of C. deserticola. The purposes of this project is to further explore the critical ecological driving and eco-strategy of two ecotypes of C. deserticola by combining the spatial molecular ecology,metabonomics, and bioinformatics tools. More specifically, exploring the ecological characteristics by Geographic Information System for Traditional Chinese Medicine（TCMGIS）and evolutionary modeling algorithm; revealing the quality characteristics using Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) technique; elaborating the effects of mutual benefit parasitic eco-adaptive suites of C.deserticola- Haloxylon ammodendron on quality variation for C. deserticola; developing of simple sequence repeat (SSR) and single nucleotide polymorphism(SNP) markers derived from transcriptome sequences and identifying the molecular geographic markers and critical driving eco-factors which related to quality variation. Our study is helpful for screening the excellent germplasm resources，selecting suitable growth location and guiding the good agricultural practices on C. deserticola. In addition, the results of this project are expected to reveal the biological essence of ecotype-variation and eco-adaptation for Dao-di herbs from a new perspective.

英文关键词： Quality evaluation;Molecular identification;Medicinal plants