南海红树秋茄内生真菌多样性及抗肿瘤活性代谢产物研究

项目名称： 南海红树秋茄内生真菌多样性及抗肿瘤活性代谢产物研究

项目编号： No.21472251

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 环境科学、安全科学

项目作者： 佘志刚

作者单位： 中山大学

项目金额： 85万元

中文摘要： 秋茄是我国天然分布最广纬度最高的红树植物, 也是我国东南沿海的主要造林树种，有极强的抗寒能力，民间将秋茄作为药物使用，具有收敛,止血，抗炎和抗菌效果。秋茄具有丰富的共生真菌，但没有得到系统研究，本课题从我国不同的红树保护区采集秋茄树叶，果实和树枝中，分离共生真菌，进行菌种鉴定和筛选，研究秋茄真菌多样性和不同生态环境对秋茄内生真菌的影响。在化学筛选和抗肿瘤药理指导下，进行实验室规模培养，分离和鉴定活性代谢产物，对代谢物进一步进行体外和体内抗肿瘤活性研究，对具有抗肿瘤活性先导化合物，进行结构修饰和构效关系研究，寻找抗肿瘤候选药物。并且从内生真菌和代谢产物的多样性，以及药理和生源关系等方面研究内生真菌和宿主之间的关系。为秋茄的生态保护和深度开发利用提供理论依据。该项目的研究对开发新的抗肿瘤海洋药物，对秋茄生态保护和深度开发利用具有重要意义和广阔前景。

中文关键词： 海洋真菌；抗肿瘤活性；次级代谢产物；构效关系；先导化合物

英文摘要： Kandelia candel, a member of the Rhizophoraceae, is the dominant coastal mangrove species and the major species used for mangrove rehabilitation along the South China coast. Kandelia candel is the most cold-tolerant mangrove species because the distribution zone of Kandelia candel is of the highest latitude comparing to other mangroves. In traditional chinese medicine,Kandelia candel shows convergence, hemostatic, anti-inflammatory and antibacterial effects. Kandelia candel endophytic fungi are rich but not yet investigated systematically.The aim of this project is to discover new antitumor metabolites from the Kandelia candel endophytic fungi, and to disclose the influence on the microorganism diversity and metabolites diversity from the ecological environment. Firstly, we will isolate and identify Kandelia candel endophytic fungi from Kandelia candel leaves, seeds and branches collected from different mangrove reserves of our country. We will carry out the research on the relationship between the microorganism diversity and the ecological environment. Secondly,Under the guidance of chemical and antitumor activities screening, the Kandelia candel endophytic fungi will be undertaken lab scale cultivation, the active metabolites will be isolated and identified.The antitumor active compounds will further undertake synthesis, structural modification and structure-activity relationship research, searching for antitumor drug candidate. This project will provide a theoretical basis for the development of ecological protection of Kandelia candel. The research is of great significance and broad prospects on the development of the new marine derived anticancer drugs and the protection of the mangrove ecological environment.

英文关键词： marine fungi;antitumor activity;metabolites;Structure-activity relationship;leading compounds